In Vitro Assays to Study Epithelial Cell Differentiation

  • Christopher W. Dawson
  • Lawrence S. Young
Part of the Methods in Molecular Biology™ book series (MIMB, volume 174)


The development of in vitro systems for the culture of human epithelial cells has aided the study of epithelial cell transformation by the epitheliotrophic papilloma-viruses (1). Although long-term proliferation of keratinocytes in vitro can now be achieved, the tissue-culture environment is not optimal for differentiation, hampering the study of virus:cell interactions in differentiating epithelial cells.


Complete Growth Medium Raft Culture Count Cell Number Epithelial Cell Transformation Keratinocyte Terminal Differentiation 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Christopher W. Dawson
    • 1
  • Lawrence S. Young
    • 1
  1. 1.CRC Institute for Cancer StudiesThe University of Birmingham Medical SchoolBirminghamUK

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