In Vitro Assays to Study Epithelial Cell Differentiation

  • Christopher W. Dawson
  • Lawrence S. Young
Part of the Methods in Molecular Biology™ book series (MIMB, volume 174)

Abstract

The development of in vitro systems for the culture of human epithelial cells has aided the study of epithelial cell transformation by the epitheliotrophic papilloma-viruses (1). Although long-term proliferation of keratinocytes in vitro can now be achieved, the tissue-culture environment is not optimal for differentiation, hampering the study of virus:cell interactions in differentiating epithelial cells.

Keywords

Glycerol Acetone EDTA Hydrocortisone Penicillin 

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Christopher W. Dawson
    • 1
  • Lawrence S. Young
    • 1
  1. 1.CRC Institute for Cancer StudiesThe University of Birmingham Medical SchoolBirminghamUK

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