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Gene Knockout Protocols pp 335–349Cite as

In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 158))

Abstract

Genetic mapping of phenotypic traits in mammals has historically relied on meiotic recombination together with the use of polymorphic markers. Some examples of these markers, in chronological order, are coat color alleles, protein polymorphisms, restriction fragment length polymorphisms (RFLP) (1,2), microsatellite repeats (simple sequence length polymorphisms, or SSLPs) (3,4), and most recently single nucleotide polymorphisms (SNPs) arrayed on chips (5). These approaches have been employed to map both single-gene mutations and genes involved in complex traits.

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Author information

Authors and Affiliations

  1. Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA

    Desmond J. Smith

Authors
  1. Desmond J. Smith
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Editor information

Editors and Affiliations

  1. National Vision Research Institute of Australia, Carlton, Victoria, Australia

    Martin J. Tymms

  2. Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia

    Ismail Kola

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© 2001 Humana Press Inc., Totowa, NJ

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Smith, D.J. (2001). In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:335

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  • DOI: https://doi.org/10.1385/1-59259-220-1:335

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-572-0

  • Online ISBN: 978-1-59259-220-3

  • eBook Packages: Springer Protocols

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