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Transgenic Studies in the Mouse

Improving the Technology Towards a Conditional Temporal and Spatial Approach

  • Protocol
Gene Knockout Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 158))

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Abstract

The biological consequences of altered gene expression as a result of gain of function mutations or gene dosage remains an important question in molecular biology and molecular medicine. This is of particular interest in medical research due to the many clinically relevant diseases that involve altered gene expression. The biology of cancer is one such example whereby a spectrum of genes may be inappropriately activated or inactivated contributing to disease progression. These genes provide ideal and direct targets for the development of cancer therapeutics and emphasize the requirement for transgenic mice as in vivo disease models. Furthermore, the inappropriate expression of transcription factor encoding genes may lead to dysregulation of gene expression and significant biological consequences. These questions can only be addressed by using technical strategies that allow the introduction or activation of a test protein in a given spatial and temporal manner. New technologies are continually being sought in order to generate sophisticated animal models that appropriately reflect the genetic basis of the disease of interest. These animals have the added benefit of being invaluable in drug discovery (1).

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  1. Centre for Functional Genomics and Human Disease, Monash University, Melbourne, Australia

    Sika Ristevski

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  1. Sika Ristevski
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Editors and Affiliations

  1. National Vision Research Institute of Australia, Carlton, Victoria, Australia

    Martin J. Tymms

  2. Centre for Functional Genomics and Human Disease, Institute of Reproduction and Development, Monash University, Clayton, Victoria, Australia

    Ismail Kola

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Ristevski, S. (2001). Transgenic Studies in the Mouse. In: Tymms, M.J., Kola, I. (eds) Gene Knockout Protocols. Methods in Molecular Biology, vol 158. Humana Press. https://doi.org/10.1385/1-59259-220-1:319

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  • DOI: https://doi.org/10.1385/1-59259-220-1:319

  • Publisher Name: Humana Press

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