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Anti-Nitrotyrosine Antibodies for Immunohistochemistry

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Septic Shock Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 36))

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Abstract

Nitrotyrosine is an important marker for the formation of peroxynitrite and possibly other reactive nitrogen species derived from nitric oxide in vivo (1). Pathological conditions can substantially increase the production of nitric oxide, yet this molecule itself does not generally yield nitration of tyrosine residues in proteins when added to biological samples (1,2). However nitric oxide reacts at near diffusion-limited rates with superoxide (O2 −) to form the strong oxidant peroxynitrite (ONOO−) (3). Nitration on the 3-position of tyrosine is a major product of peroxynitrite attack on proteins (4,5). Certainly, small amounts of nitrotyrosine can be produced in vivo by other mechanisms (6), but peroxynitrite is by far the most efficient mechanism for nitrating tyrosine under biologically relevant conditions with natural antioxidants and alternative targets present.

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Author information

Authors and Affiliations

  1. Department of Anesthesiology and The UAB Center for Free Radical Biology, The University of Alabama at Birmingham, Birmingham, AL

    Liliana Viera & Yao Zu Ye

  2. Departamento de Histologia y Embriologia, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay

    Liliana Viera

  3. Departments of Anesthesiology, Biochemistry and Molecular Genetics, and Neurobiology and The UAB Center for Free Radical Biology, The University of Alabama at Birmingham, Birmingham, AL

    Joseph S. Beckman

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  1. Liliana Viera
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  2. Yao Zu Ye
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Editors and Affiliations

  1. Imperial College School of Medicine, London, UK

    Thomas J. Evans

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© 2000 Humana Press Inc.

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Viera, L., Ye, Y.Z., Beckman, J.S. (2000). Anti-Nitrotyrosine Antibodies for Immunohistochemistry. In: Evans, T.J. (eds) Septic Shock Methods and Protocols. Methods in Molecular Medicine™, vol 36. Humana Press. https://doi.org/10.1385/1-59259-216-3:159

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  • DOI: https://doi.org/10.1385/1-59259-216-3:159

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-730-4

  • Online ISBN: 978-1-59259-216-6

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