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Assay for Oxidative Metabolism

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Immunocytochemical Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 115))

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Abstract

Phagocytic leukocytes that are exposed to opsonized particles, chemoattractants, or selected cytokines undergo a rapid burst in oxygen consumption and activation of the enzyme responsible for the oxidative metabolic burst, NADPH oxidase (reviewed in ref. 1). Active NADPH oxidase catalyzes the reaction:

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Author information

Authors and Affiliations

  1. Division of Hematology, Food and Drug Administration Center for Biologics Evaluation & Research, Bethesda, MD

    Liana Harvath

  2. Applied Information Technology Food & Drug Administration, Center for Biologics Evaluation & Research, Bethesda, MD

    Douglas A. Terle

Authors
  1. Liana Harvath
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  2. Douglas A. Terle
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Editor information

Editors and Affiliations

  1. Catholic University of America, Washington, DC

    Lorette C. Javois

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© 1999 Humana Press Inc., Totowa, NJ

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Harvath, L., Terle, D.A. (1999). Assay for Oxidative Metabolism. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:309

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  • DOI: https://doi.org/10.1385/1-59259-213-9:309

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-813-4

  • Online ISBN: 978-1-59259-213-5

  • eBook Packages: Springer Protocols

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