Abstract
Phagocytic leukocytes play a major role in host defense because they rapidly migrate to sites of infection and destroy invading microorganisms. Specific signal molecules (chemoattractants), released by bacteria or endogenously generated by the host, can elicit directed leukocyte migration (chemotaxis) to the inflammatory site. Chemotaxis is initiated by the specific interaction (binding) of chemoattractants with leukocyte plasma membrane receptors. Polymorphonuclear leukocytes (neutrophils) migrate to a variety of chemoattractants, including N-formyl peptides, complement-derived C5a, leukotriene B4, interleukin-8, and platelet-activating factor, each of which has a distinct receptor on the leukocyte plasma membrane.
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© 1999 Humana Press Inc.
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Harvath, L., Aksamit, R.R., Cunningham, R.E. (1999). Assay for Chemoattractant Binding. In: Javois, L.C. (eds) Immunocytochemical Methods and Protocols. Methods in Molecular Biology™, vol 115. Humana Press. https://doi.org/10.1385/1-59259-213-9:299
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DOI: https://doi.org/10.1385/1-59259-213-9:299
Publisher Name: Humana Press
Print ISBN: 978-0-89603-813-4
Online ISBN: 978-1-59259-213-5
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