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Overview of Automated Immunostainers

  • Gilbert E. Herman
  • Edna A. Elfont
  • Alton D. Floyd
Part of the Methods in Molecular Biology™ book series (MIMB, volume 115)

Abstract

A significant consideration in the use of immunoreagents for microscopic slide staining is their cost. For many antibodies, fractions of a milliliter of fluid may be very expensive, particularly in the undiluted form. Traditional staining methods frequently use more reagent than allowed for by supply budget constraints. Additionally, routine immunostaining requires several dozen changes of reagent and is therefore labor-intensive, particularly when large numbers of slides must be stained. Using manual staining methods, wash procedures are such that they may result in slide to slide variations. Drying artifact is difficult to avoid, and occasionally, a slide may not even receive a reagent.

Keywords

Slide Surface Evaporative Loss Spray Pattern Light Blue Color Reagent Carryover 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Gilbert E. Herman
    • 1
  • Edna A. Elfont
    • 1
  • Alton D. Floyd
    • 2
  1. 1.Histopathology AssociatesWest Bloomfield
  2. 2.Consultant in BiotechnologyEdwardsburg

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