Biotinylation of Antibodies
The high affinity and specificity of the avidin-biotin interaction permit diverse applications in immunology, histochemistry, in situ hybridizations, affinity chromatography, and many other areas (1) (see Chapters 25 and 26). It was first exploited in immunocytochemical applications in the mid-1970s (2,3), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to the sample, and the detection is accomplished by using labeled avidin. Avidin with a variety of labels are available commercially, including fluorescent, enzyme, iodine, ferritin, or gold labels.
KeywordsSodium Azide Gold Label Sodium Borate Buffer Charge Cell Surface Increase Protein Concentration
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