Biotinylation of Antibodies

  • Su-Yau Mao
Part of the Methods in Molecular Biology™ book series (MIMB, volume 115)

Abstract

The high affinity and specificity of the avidin-biotin interaction permit diverse applications in immunology, histochemistry, in situ hybridizations, affinity chromatography, and many other areas (1) (see Chapters 25 and 26). It was first exploited in immunocytochemical applications in the mid-1970s (2,3), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to the sample, and the detection is accomplished by using labeled avidin. Avidin with a variety of labels are available commercially, including fluorescent, enzyme, iodine, ferritin, or gold labels.

Keywords

Hydrolysis Carbohydrate Iodine Amide Glycine 

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Su-Yau Mao
    • 1
  1. 1.Medimmune, Inc.Gaithersburg

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