Abstract
Experimental studies that investigate the functional and mechanistic properties of an imprinted locus require material in which the two parental chromosome homologs can be easily distinguished. The use of animals with uniparental duplications and deficiencies of imprinted regions of interest is one powerful approach. This material not only allows the successful analysis of the monoallelic expression and genome modifications associated with imprinting, but also is useful for studying the developmental roles of imprinted genes through the analysis of conceptuses in which the dosage of imprinted genes has been perturbed (1-3).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cattanach, B. M. and Kirk, M. (1985) Differential activity of maternally and paternally derived chromosome regions in mice. Nature 315, 496–498.
Ferguson-Smith, A. C., Cattanach, B. M., Barton, S. C., Beechey, C. V., and Surani, M. A. (1991) Embryological and molecular investigations of parental imprinting on mouse chromosome 7. Nature 351, 667–670 (1991).
McLaughlin, K. J., Szabo, P., Haegel, H., and Mann, J. R. (1996) Mouse embryos with paternal duplication of an imprinted chromosome 7 region die at midgestation and lack placental spongiotrophoblast. Development 122, 265–270.
Cattanach, B. M. (1986) Parental origin effects in mice. J. Embryol. Exp. Morphol. 97 (Suppl.), 137–150.
Ledbetter, D. H. and Engel, E. (1995) Uniparental disomy in humans: development of an imprinting map and its implications for prenatal diagnosis. Human Mol. Genet. 4, 1757–1764.
Joyce, J. and Ferguson-Smith, A. C. (1999) Genomic imprinting in development and disease, in Developmental Genetics, Epigenetics and Environmental Regulation (V. Russo, D. Cove, L. Edgar, R. Jaenisch, and F. Salamani, eds.), Springer-Verlag, Berlin, pp.421–434.
Cattanach, B. M. and Beechey, C. V. (1997) Genomic imprinting in the mouse: possible final analysis, in Genomic Imprinting: Frontiers in Molecular Biology (Reik, W. and Surani, M. A., eds.), IRL, Oxford, U.K., pp.118–141.
Oakey, R. J., Matteson, P. G., Litwin, S., Tilghman, S. M., and Nussbaum, R. L. (1995) Nondisjunction rates and abnormal embryonic development in a mouse cross between heterozygotes carrying a (7, 18) Robertsonian translocation chromosome. Genetics 141, 667–674.
Beechey, C. V. and Evans, E. P (1996) Chromosomal variants-numerical variants and structural rearrangements, in Genetic Variants and Strains of the Laboratory Mouse, Vol. 2 (Lyon, M. F., Rastan, S., and Brown, S. D. M., eds.), Oxford University Press, Oxford, pp.1452–1506.
Georgiades, P., Watkins, M., Surani, M. A., and Ferguson-Smith, A. C. (2000). Parental origin specific developmental defects in mice with uniparental disomy for chromosome 12. Development 127, 4719–4728.
Epstein, C. J. (1986) Generation and properties of mouse aneuploids, in The Consequences of Chromosome Imbalance, Cambridge University Press, Cambridge, chapter 10.
Gropp, A., Giers, D., and Kolbus, U. (1974). Trisomy in the fetal backcross progeny of male and female metacentric heterozygotes of the mouse. I. Cytogenet. Cell Genet. 13, 511–535.
Magnuson, T., Debrot, S., Dimpfl, J., Zweig, A., Zamora, T., and Epstein, C. J. (1985) The early lethality of autosomal monosomy in the mouse. J. Exp. Zool. 236, 353–360.
Williamson, C. M., Schofield, J., Dutton, E. R., Seymour, A., Beechey, C. V., Edwards, Y. H., and Peters, J. (1996) Glomerular-specific imprinting of the mouse gsalpha gene: how does this relate to hormone resistance in albright hereditary osteodystrophy? Genomics 36, 280–287.
Searle, A. G. and Beechey, C. V. (1990) Genome imprinting phenomena on mouse chromosome 7. Genet. Res. 56, 237–244.
Ferguson-Smith, A. C., Sasaki, H., Cattanach, B. M., and Surani, M. A. (1993) Parental origin specific epigenetic modification of the mouse H19 gene. Nature 362, 751–755.
Eversole-Cire, P., Ferguson-Smith, A. C., Sasaki, H., Brown, K., Cattanach, B. M., Gonzales, F. A., Surani, M. A., and Jones, P. A. (1993) Induced activation of an imprinted Igf2 gene in mouse somatic cell cultures. Mol. Cell. Biol. 13, 4928–4938.
Eversole-Cire P., Ferguson-Smith, A. C., Surani, M. A., and Jones, P. A. (1995) Coordinate regulation of Igf2 and H19 in cultured cells. Cell Growth Differentiation 6, 337–345.
McLaughlin, K. J., Kochanowski, H., Solter, D., Schwarzkopf, G., Szabo, P. E., and Mann, J. R. (1997) Roles of the imprinted gene Igf2 and paternal duplication of distal chromosome 7 in the perinatal abnormalities of androgenetic mouse chimeras. Development 124, 4897–4904.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Ferguson-Smith, A.C., Tevendale, M., Georgiades, P., Grandjean, V. (2002). Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome. In: Ward, A. (eds) Genomic Imprinting. Methods in Molecular Biology™, vol 181. Humana Press. https://doi.org/10.1385/1-59259-211-2:41
Download citation
DOI: https://doi.org/10.1385/1-59259-211-2:41
Publisher Name: Humana Press
Print ISBN: 978-0-89603-741-0
Online ISBN: 978-1-59259-211-1
eBook Packages: Springer Protocols