Detecting Hyaluronidase and Hyaluronidase Inhibitors

Hyaluronan-Substrate Gel and -Inverse Substrate Gel Techniques
  • Kazuhiro Mio
  • Antonei B. Csóka
  • Susan Nawy Stair
  • Robert Stern
Part of the Methods in Molecular Biology™ book series (MIMB, volume 171)


Hyaluronidases are a group of enzymes that degrade the glycosaminoglycan hyaluronan (HA, hyaluronic acid). Many types of hyaluronidases are reported, from prokaryotes to eukaryotes (1,2). These enzymes have a wide variety of properties, including substrate specificity, inhibitor sensitivity, and a range of pH optima. Streptomyces hyaluronidase, and the venom hyaluronidases from bee, snake, and scorpion are active at neutral pH. Hyal-1, the best-characterized somatic hyaluronidase (3, 4, 5), product of one of the six hyaluronidase-like sequences in the human genome (6), is an acid-active enzyme with an optimum at pH 3.7. The sperm-specific PH-20 (7) has apparently two pH optima, pH 4.5 and 7.5, resulting possibly from two forms of the enzyme, membrane-bound and soluble (8,9)


Sodium Dodecyl Sulfate Alcian Blue Coomassie Blue Staining Alcian Blue Staining Bromphenol Blue 
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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Kazuhiro Mio
  • Antonei B. Csóka
  • Susan Nawy Stair
  • Robert Stern

There are no affiliations available

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