Disaccharide Composition in Glycosaminoglycans/Proteoglycans Analyzed by Capillary Zone Electrophoresis

  • Nikos K. Karamanos
  • Anders Hjerpe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 171)


During the past decade, the use of fully automated equipment for capillary electrophoresis (CE) has made this a routine method for the study of soluble analytes. The separation of such compounds in capillaries (20–200 µm id) and in strong electric fields (around 50 kV/m) seems to be exceptionally efficient for separating both large and small molecules. The most common type of CE is capillary zone electrophoresis (CZE), which is done with the separation buffer free in an otherwise empty capillary. These separations, in principle, share some features not only with gel electrophoresis but also with high-performance liquid chromatography (HPLC), and they provide a unique combination of both analytical techniques. CZE is therefore an alternative to HPLC and it is sometimes advantageous because there are no problems related to laminar flow and other wall effects.


Heparan Sulfate Chondroitin Sulfate Uronic Acid Capillary Zone Electrophoresis Dermatan Sulfate 
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  1. 1.
    Karamanos, N. K., Axelsson, S., Vanky, P., Tzanakakis, G. N., and Hjerpe, A. (1995) Determination of hyaluronan-and galactosaminoglycan-derived disaccharides by high-performance capillary electrophoresis at the attomole level. Applications to analyses of tissue and cell culture proteoglycans. J. Chromatogr. A 696(2), 295–305.PubMedCrossRefGoogle Scholar
  2. 2.
    Lamari, F., Theocharis, A., Hjerpe, A., and Karamanos, N. K. (1999) Ultrasensitive capillary electrophoresis of sulfated disaccharides in chondroitin/dermatan sulfates by laser-induced fluorescence after derivatization with 2-aminoacridone. J. Chromatogr. B. 730, 129–133.CrossRefGoogle Scholar
  3. 3.
    Lamari, F. and Karamanos, N. K. (1999) High-performance capillary electrophoresis as a powerful analytical tool of glycoconjugates. J. Liq. Chromatogr. Rel. Technol. 22, 1295–1317.CrossRefGoogle Scholar
  4. 4.
    Karamanos, N. K. (1999) Proteoglycans: biological roles and strategies for isolation and determination of their glycan constituents, in Proteome and Protein Analysis (Kamp, M., Kyriakides, D., and Choli-Papadopoulou, T. eds.), Springer-Verlag, Heidelberg, Germany, pp. 341–363.Google Scholar
  5. 5.
    Karamanos, N. K. and Hjerpe, A. (1998) A survey of methodological challenges for glycosaminoglycan/proteoglycan analysis and structural characterization by capillary electrophoresis. Electrophoresis 19, 2561–2571.PubMedCrossRefGoogle Scholar
  6. 6.
    Karamanos, N. K. and Hjerpe, A. (1999) Strategies for analysis and structure characterization of glycan/proteoglycans by capillary electrophoresis. Their diagnostic and biopharmaceutical importance. Biomed. Chromatogr. 13, 507–512.PubMedCrossRefGoogle Scholar
  7. 7.
    Karamanos, N. K., and Hjerpe, A. (1997) High-performance capillary electrophoretic analysis of hyaluronan in effusions from human malignant mesothelioma. J. Chromotogr. B. 696,277–281.CrossRefGoogle Scholar
  8. 8.
    Karamanos, N. K., Vanky, P., Tzanakakis, G. N., and Hjerpe, A. (1996) High-performance capillary electrophoresis method to characterize heparin and heparan sulfate disaccharides. Electrophoresis 17, 391–395.PubMedCrossRefGoogle Scholar
  9. 9.
    Karamanos, N. K., Vanky, P., Tzanakakis, G. N., Tsegenidis, T., and Hjerpe, A. (1997) High-performance liquid chromatography for determining disaccharide composition in heparin and heparan sulfate. J. Chromatogr. A 765, 169–179.PubMedCrossRefGoogle Scholar
  10. 10.
    Plaas, A. H., West, L. A., Wong-Palms, S., and Nelson, F. R. (1998) Glycosaminoglycan sulfation in human osteoarthritis. Disease-related alterations at the non-reducing termini of chondroitin and dermatan sulfate. J. Biol. Chem. 273, 12,642–12,649.PubMedCrossRefGoogle Scholar
  11. 11.
    Plaas, A. H., Wong-Palms, S., Roughley, P. J., Midura, R. J., and Hascal, V. C. (1997) Chemical and immunological assay of the nonreducing terminal residues of chondroitin sulfate from human aggrecan. J. Biol. Chem. 272(33), 20,603–20,610.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Nikos K. Karamanos
    • 1
  • Anders Hjerpe
    • 2
  1. 1.Department of ChemistryUniversity of PatrasPatrasGreece
  2. 2.Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Division of Pathology, Karolinska InstituteHuddinge University HospitalHuddingeSweden

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