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Disaccharide Composition in Glycosaminoglycans/Proteoglycans Analyzed by Capillary Zone Electrophoresis

  • Nikos K. Karamanos
  • Anders Hjerpe
Part of the Methods in Molecular Biology™ book series (MIMB, volume 171)

Abstract

During the past decade, the use of fully automated equipment for capillary electrophoresis (CE) has made this a routine method for the study of soluble analytes. The separation of such compounds in capillaries (20–200 µm id) and in strong electric fields (around 50 kV/m) seems to be exceptionally efficient for separating both large and small molecules. The most common type of CE is capillary zone electrophoresis (CZE), which is done with the separation buffer free in an otherwise empty capillary. These separations, in principle, share some features not only with gel electrophoresis but also with high-performance liquid chromatography (HPLC), and they provide a unique combination of both analytical techniques. CZE is therefore an alternative to HPLC and it is sometimes advantageous because there are no problems related to laminar flow and other wall effects.

Keywords

Heparan Sulfate Chondroitin Sulfate Uronic Acid Capillary Zone Electrophoresis Dermatan Sulfate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Nikos K. Karamanos
    • 1
  • Anders Hjerpe
    • 2
  1. 1.Department of ChemistryUniversity of PatrasPatrasGreece
  2. 2.Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Division of Pathology, Karolinska InstituteHuddinge University HospitalHuddingeSweden

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