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Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates

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DNA-Protein Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 148))

Abstract

Atomic force microscopy (AFM; also called scanning force microscopy [SFM]) is a rather novel technique that offers unique advantages in the potential for the very high resolution of DNA and small ligands in the absence of stains, shadows, and labels (1,2). Furthermore, the scanning can be performed in air or liquid. The latter is particularly important for resolving fully hydrated structures. The AFM is theoretically capable of resolving structural details at the level of atomic dimensions, provided that the specimen is not dynamic.

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General Reading

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Author information

Authors and Affiliations

  1. Departments of Biology and Microbiology, Arizona State University, Tempe, AZ

    Yuri L. Lyubchenko

  2. Seattle Genetics, Bothell, WA

    Alexander A. Gall

  3. Departments of Plant Biology and Microbiology, Arizona State University, Tempe, AZ

    Luda S. Shlyakhtenko

Authors
  1. Yuri L. Lyubchenko
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  2. Alexander A. Gall
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  3. Luda S. Shlyakhtenko
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Editor information

Editors and Affiliations

  1. Centre de Recherche en Cancérologie de l’Université Laval, Centre Hopital Universitaire de Québec et Départment de biologie médicale, Université Laval, Québec, QC, Canada

    Tom Moss

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© 2001 Humana Press Inc., Totowa, NJ

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Lyubchenko, Y.L., Gall, A.A., Shlyakhtenko, L.S. (2001). Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates. In: Moss, T. (eds) DNA-Protein Interactions. Methods in Molecular Biology, vol 148. Humana Press. https://doi.org/10.1385/1-59259-208-2:569

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  • DOI: https://doi.org/10.1385/1-59259-208-2:569

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-625-3

  • Online ISBN: 978-1-59259-208-1

  • eBook Packages: Springer Protocols

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