Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates

  • Yuri L. Lyubchenko
  • Alexander A. Gall
  • Luda S. Shlyakhtenko
Part of the Methods in Molecular Biology book series (MIMB, volume 148)


Atomic force microscopy (AFM; also called scanning force microscopy [SFM]) is a rather novel technique that offers unique advantages in the potential for the very high resolution of DNA and small ligands in the absence of stains, shadows, and labels (1,2). Furthermore, the scanning can be performed in air or liquid. The latter is particularly important for resolving fully hydrated structures. The AFM is theoretically capable of resolving structural details at the level of atomic dimensions, provided that the specimen is not dynamic.


Plastic Syringe Mica Surface Fluid Cell MultiMode System Nucleoprotein Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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General Reading

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Yuri L. Lyubchenko
    • 1
  • Alexander A. Gall
    • 2
  • Luda S. Shlyakhtenko
    • 3
  1. 1.Departments of Biology and MicrobiologyArizona State UniversityTempe
  2. 2.Seattle GeneticsBothell
  3. 3.Departments of Plant Biology and MicrobiologyArizona State UniversityTempe

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