Ultraviolet-Laser Footprinting

  • Johannes Geiselmann
  • Frederic Boccard
Part of the Methods in Molecular Biology book series (MIMB, volume 148)

Abstract

A large number of processes within the cell, in particular the regulation of gene expression, rely on the binding of proteins to specific sites on the DNA. A primary ingredient to understanding these processes is the characterization of the protein-DNA interaction (1). Such a characterization consists in determining the position of the binding site on the DNA and measuring the affinity of the protein for this recognition site. A wide variety of footprinting techniques can accomplish this task (2,3).

Keywords

Glycerol Phenol Urea Electrophoresis MgCl2 

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Johannes Geiselmann
    • 1
  • Frederic Boccard
    • 2
  1. 1.Plasticité et Expression des Génomes MicrobiensUniversité Joseph FourierGrenobleFrance
  2. 2.Centre de Génétique MoléculaireCNRSYvetteFrance

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