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Footprinting with Exonuclease III

  • Willi Metzger
  • Hermann Heumann
Part of the Methods in Molecular Biology book series (MIMB, volume 148)

Abstract

Within the last few years footprinting techniques have become increasingly important in the study of protein-nucleic acid interactions. This is partly the result of a fast-growing number of known nucleic acid-binding proteins but also because of an increase in the available probes that can be chosen in order to tackle a specific problem. There are two major groups of probes—the chemical probes and the enzymatic probes. The enzymatic probes, such as DNase I or exonuclease III, have the advantage of acting specifically on the DNA. Chemical probes are often less specific and may also react with the protein, possibly disturbing the correct interaction of protein with DNA. For the study of very fragile protein-DNA complexes, enzymatic probes are therefore often preferable.

Keywords

Ammonium Persulfate SpeedVac Concentrator Acrylamide Solution Enzymatic Probe Footprinting Technique 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Willi Metzger
    • 1
  • Hermann Heumann
    • 2
  1. 1.Ministerium für Umwelt und Forsten des Landes Rheinland-PfalzMainzGermany
  2. 2.Max Planck Institute of BiochemistryMartinsriedGermany

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