Footprinting with Exonuclease III
Within the last few years footprinting techniques have become increasingly important in the study of protein-nucleic acid interactions. This is partly the result of a fast-growing number of known nucleic acid-binding proteins but also because of an increase in the available probes that can be chosen in order to tackle a specific problem. There are two major groups of probes—the chemical probes and the enzymatic probes. The enzymatic probes, such as DNase I or exonuclease III, have the advantage of acting specifically on the DNA. Chemical probes are often less specific and may also react with the protein, possibly disturbing the correct interaction of protein with DNA. For the study of very fragile protein-DNA complexes, enzymatic probes are therefore often preferable.
KeywordsAmmonium Persulfate SpeedVac Concentrator Acrylamide Solution Enzymatic Probe Footprinting Technique
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