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Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons

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Protocols for Neural Cell Culture

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The compartmented culture method has numerous features that make it useful for studies of nerve fiber growth, among which are:

  1. 1.

    Distal axons can be exposed to a different fluid and cellular environment than cell bodies and proximal axons, which is useful for studies of trophic, ionic, and pharmacological regulation of growth.

  2. 2.

    Distal axons can be removed (neuritotomy), and subsequently regenerate, which permits many useful approaches to the study of axon growth and regeneration.

  3. 3.

    Axons can be chronically electrically stimulated during growth and regeneration.

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Further Reading

  • Campenot, R. B. (1979), Independent control of the local environments of somas and neurites, in: Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 302–307.

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  • Campenot, R. B. (1982), Development of sympathetic neurons in compartmentalized cultures I. Local control of neurite growth by nerve growth factor. Dev. Bio I. 93, 1–12.

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  • Campenot, R. B. (1992), Compartmented culture analysis of nerve growth, in: Cell-Cell Interactions: A Practical Approach, Stevenson, B., Paul, D., and Gallin, W., eds., IRL, Oxford, UK, pp. 275–298.

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  • Campenot, R. B. and Draker, D. D. (1989), Growth of sympathetic nerve fibers in culture does not require extracellular calcium. Neuron 3, 733–743.

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  • Hawrot, E. and Patterson, P. H. (1979), Long-term cultures of dissociated sympathetic neurons, in: Methods in Enzymology, vol. 28, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 574–584.

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Author information

Authors and Affiliations

  1. Department of Cell Biology, College of Medicine, University of Alberta, Edmonton, Alberta, Canada

    Robert B. Campenot & Grace Martin

Authors
  1. Robert B. Campenot
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  2. Grace Martin
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Editor information

Editors and Affiliations

  1. Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Sergey Fedoroff  & Arleen Richardson  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Campenot, R.B., Martin, G. (2001). Construction and Use of Compartmented Cultures for Studies of Cell Biology of Neurons. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:49

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  • DOI: https://doi.org/10.1385/1-59259-207-4:49

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-902-5

  • Online ISBN: 978-1-59259-207-4

  • eBook Packages: Springer Book Archive

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