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Quantification of Cells in Culture

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Cell enumeration using the hemocytometer is applicable when determining the number of cells in a suspension, and when the number of samples to be analyzed is relatively small. Hemocytometry is also useful for determining the proportion of singly dispersed cells in a suspension, and for estimating the frequency of viable cells.


  • Counting Chamber
  • Tritiated Thymidine
  • Multiwell Plate
  • Nonviable Cell
  • Scintillation Solution

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  • DOI: 10.1385/1-59259-207-4:333
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Further Reading

  • Bainbridge, D. R. and Macey, M. M. (1983), Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence. J. Immunol. Methods 62, 193–195.

    PubMed  CrossRef  CAS  Google Scholar 

  • Baserga, R. (1989), Measuring parameters of growth, in Cell Growth and Division, Baserga, R., ed., IRL, New York.

    Google Scholar 

  • Branch, D. R. and Guilbert, L. J. (1986), Practical in vitro assay systems for the measurement of hematopoietic growth factors J. Tissue Culture Methods 10, 101–108.

    CrossRef  CAS  Google Scholar 

  • Hamilton, L. H. (1956), Errors in blood cell counting. 1. Technical errors. 2. Statistical errors. Can. J. Med. Tech. 18, 8–14.

    CAS  Google Scholar 

  • Hanks, J. H. and Wallace, R. E. (1958), Determination of cell viability. Proc. Soc. Exp. Biol Med. 98, 188–192.

    PubMed  CAS  Google Scholar 

  • Harris, M. (1959), Growth measurements on monolayer cultures with an electronic cell counter. Can. Res. 19, 1020–1024.

    CAS  Google Scholar 

  • Jones, K. H. and Senft, J. A. (1985), An improved method to determine cell viability by simultaneous staining with fluorescein diacetate propidium iodide. J. Hist. Cytol. 33, 77–79.

    CAS  Google Scholar 

  • Kaltenbach, J. P., Kaltenbach, M. H., and Lyons, W. B. (1958), Nigrosin as a dye for differentiating live and dead ascites cells. Exp. Cell Res. 15, 112–117.

    PubMed  CrossRef  CAS  Google Scholar 

  • Latt, S. A. and Stetten, G. (1976), Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis. J. Histochem. Cytochem. 24, 24–33.

    PubMed  CAS  Google Scholar 

  • Phillips, H. J. and Terryberry, J. E. (1957), Counting actively metabolizing tissue cultured cells. Exp. Cell Res. 13, 341–347.

    PubMed  CrossRef  CAS  Google Scholar 

  • Schrek, R. (1944), Studies in vitro on the physiology of normal and of cancerous cells. II. The survival and the glycolysis of cells under anaerobic conditions. Arch. Pathol. 37, 319–327.

    CAS  Google Scholar 

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© 2001 Humana Press Inc., Totowa, NJ

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Richardson, A., Fedoroff, S. (2001). Quantification of Cells in Culture. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press.

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  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-902-5

  • Online ISBN: 978-1-59259-207-4

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