Abstract
The study of glial cell development and function has been considerably enhanced by the development of methods to culture oligodendrocytes and astrocytes from central nervous system tissue. A primary mixed glial culture, composed mostly of astrocytes, oligodendrocytes, and microglia, is obtained when newborn disaggregated cerebral brain cells from rat are plated at high cell density (2 × 105/cm2) in serum-supplemented medium (McCarthy and de Vellis, 1980).
Keywords
- Glial Fibrillary Acidic Protein
- Cerebral Hemisphere
- Central Nervous System Tissue
- Olfactory Lobe
- Median Fissure
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Further Reading
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© 2001 Humana Press Inc., Totowa, NJ
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Cole, R., de Vellis, J. (2001). Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:117
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DOI: https://doi.org/10.1385/1-59259-207-4:117
Publisher Name: Humana Press
Print ISBN: 978-0-89603-902-5
Online ISBN: 978-1-59259-207-4
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