Tips for Tissue Culture

  • Arleen Richardson
  • Sergey Fedoroff


Cells attach to the culture substratum with varying degrees of adherence (see  Chapter 18). Cells that must attach to the substratum are referred to as “adhering cells” (anchorage-dependent cells). Cells that do not adhere and grow as cell suspensions in the medium are referred to as “nonadhering cells” (nonanchorage-dependent cells).


Water Purification System Soybean Trypsin Inhibitor Laminar Flow Hood Rubber Policeman Tissue Culture Laboratory 
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Further Reading

  1. Bottenstein, J., Hayashi, I., Hutchings, S., Masui, J., Mather, J., McClure, D. B., Ohasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R. (1979), The growth of cells in serum-free hormone-supplemented media, in: Methods in Enzymology Cell Culture, Jakoby, W. B. and Pastan, I. H., eds., Academic, New York, pp. 94–109.Google Scholar
  2. Fogh, J., ed. (1973), Contamination in Tissue Culture. Academic, New York, pp. 1–288.Google Scholar
  3. Gabler, R., Hegde, R., and Hughes, D. (1983), Degradation of high purity water on storage. J. Liquid Chromatog. 6, 2565–2570.CrossRefGoogle Scholar
  4. Goule, M. C. (1984), Endotoxin in vertebrate cell culture: its measurement and significance, in: Uses and Standardization of Vertebrate Cell Cultures, Tissue Culture Association, Gaithersburg, MD 5, 125–136.Google Scholar
  5. Hay, R. J., Caputo, J., and Macy, M. L., eds. (1992), Testing for microbial contaminants. in: ATCC Quality Control Methods for Cell Lines, American Type Culture Collection, Rockville, MD, pp. 19–48.Google Scholar
  6. MacLeod, R. A. F., Dirks, W. G., Matsuo, Y., Kaufmann, M., Milch, H., and Drexler, H. G. (1999), Widespread intraspecies cross-contamination of human tumor cell lines arising at source. Int. J. Cancer 83, 555–563.PubMedCrossRefGoogle Scholar
  7. Mather, J., Kaczarowski, F., Gabler, R., and Wilkins, F. (1986), Effects of water purity and addition of common water contaminants on the growth of cells in serum-free media. BioTechniques 4, 56–63.Google Scholar
  8. McKeehan, W. L. (1977), The effect of temperature during trypsin treatment on viability and multiplication of single normal human and chicken fibroblasts. Cell Biol. Int. Reports 1, 335–343.CrossRefGoogle Scholar
  9. Melnick, J. L. and Wallis, C. (1977), Problems related to the use of serum and trypsin in the growth of monkey kidney cells, in: Developments in Biological Standardization, Perkins, F. T. and Regamey, R. H., eds., S. Karger, Basel, pp. 77–82.Google Scholar
  10. Perkins, J. J. (1969), Principles and Methods of Sterilization in Health Sciences. Charles C. Thomas, Springfield, IL, pp. 1–549.Google Scholar
  11. Perlman, D. (1979), Use of antibiotics in cell culture media. in: Methods in Enzymology: Cell Culture, vol. 58, Jacoby, W. B. and Pasten, I. H., eds., Academic, New York, pp. 110–116.Google Scholar
  12. Rottem, S. and Barile, M. F. (1993), Beware of mycoplasmas. Trends Biotechnol. 11, 143–150.PubMedCrossRefGoogle Scholar
  13. Ryan, J. A. (1994), Understanding and managing cell culture contamination. Corning, Technical Publication TC-CI-559, Corning, NY.Google Scholar
  14. Taylor, W. G. (1984), Toxicity and hazards to successful culture: cellular responses to damage induced by light, oxygen or heavy metals, in: Uses and Standardization of Vertebrate Cell Cultures, Patterson, M. K., ed., Tissue Culture Association, Gaithersburg, MD, 58–70.Google Scholar
  15. Wang, R. J. and Nixon, T. (1978), Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light. In Vitro 14, 715–722.PubMedCrossRefGoogle Scholar
  16. Waymouth, C. (1982), Methods for obtaining cells in suspension from animal tissues, in: Cell Separation Methods and Selected Applications, vol. 1, Pretlow, T. G. and Pretlow, T. P., eds., Academic, New York, 1, 1–29.Google Scholar
  17. Waymouth, C. (1993), Tissue Dissociation Guide, Worthington Biochemical, Freehold, NJ, pp. 1–78.Google Scholar
  18. Zigler, J. S., Lepe-Zuniga, J. L., Vistica, B., and Gery, I. (1985), Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium. In Vitro Cell. Dev. Biol. 21, 282–287.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Arleen Richardson
    • 1
  • Sergey Fedoroff
    • 1
  1. 1.Department of Anatomy and Cell Biology, College of MedicineUniversity of SaskatchewanSaskatoonCanada

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