Applications of Electron Microscopy for Studying Protein-DNA Complexes
Electron microscopic (EM) studies of DNA-protein complexes have led to many insights into biological reactions. There are three general methods that can be used to great advantage in such studies, each gives a different type of information, but each has its own disadvantages. In this chapter, we will discuss these methods and show examples of the usefulness of each.
KeywordsCarbon Film Alcian Blue Direct Adsorption Electron Microscopic Grid Capillary Pipet
- 6.Webb, B. L., Cox, M. M., and Inman, R. B. (1997) Recombinational DNA repair: the RecF and RecR proteins limit the extension of RecA filaments beyond singlestrand DNA gaps. Gene 91, 1–20.Google Scholar