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Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol

  • Rick T. Giberson
  • Richard S. DemareeJr.
Part of the Methods in Molecular Biology™ book series (MIMB, volume 117)

Abstract

The use of microwave energy to reduce processing times during fixation for electron microscopy (EM) is well established in the literature (1-5). The microwave protocol described in this chapter is based on the work of Giberson et al. (6,7) and differs from the microwave literature in that each step (fixation, dehydration, embedding, and resin polymerization) in specimen processing is done in the microwave. The time savings gained from this approach are considerable and the results, based on our experience and that of others (8,9), are equal or better than those achieved from routine processing protocols.

Keywords

Microwave Irradiation Microcentrifuge Tube Propylene Oxide Sodium Cacodylate Cold Spot 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1999

Authors and Affiliations

  • Rick T. Giberson
    • 1
  • Richard S. DemareeJr.
    • 2
  1. 1.Ted Pella, IncRedding
  2. 2.Department of Biological SciencesCalifornia State UniversityChico

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