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Detection and Analysis of Synaptosis

  • Protocol
Apoptosis Techniques and Protocols

Part of the book series: Neuromethods ((NM,volume 37))

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Abstract

Essentially three modes of synaptic elimination have been described (1): (1) loss of synapses in synaptically connected neurons following physiological neuron death during development or hormonally driven reorganization; (2) process (generally axonal) retraction and proteolytic degradation of presynaptic elements involving lysosomal upregulation and autophagy; (3) elimination of intact nerve terminals by glial phagocytosis (“synaptic stripping”), notably in the facial nucleus as a retrograde transneuronal effect following axotomy-here microglial cells play a major role (2,3). Similar phagocytosis of intact terminals can be performed by protoplasmic astrocytes as described below.

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Author information

Authors and Affiliations

  1. Departments of Medicine and Neurology, University of Medicine, Los Angeles, CA

    Greg M. Cole PHD

  2. Departments of Medicine and Neurology, VAGLAHS, North Hills, CA

    Karen Gylys PHD

  3. Department of Nursing, University of California at Los Angeles, Los Angeles, CA

    Greg M. Cole PHD

  4. Department of Nursing, VAGLAHS, North Hills, CA

    Karen Gylys PHD

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  1. Greg M. Cole PHD
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Editors and Affiliations

  1. Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada

    Andréa C. LeBlanc

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Cole, G.M., Gylys, K. (2002). Detection and Analysis of Synaptosis. In: LeBlanc, A.C. (eds) Apoptosis Techniques and Protocols. Neuromethods, vol 37. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-188-4:177

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  • DOI: https://doi.org/10.1385/1-59259-188-4:177

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-1-58829-012-0

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