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Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs

  • Protocol
Apoptosis Techniques and Protocols

Part of the book series: Neuromethods ((NM,volume 37))

Abstract

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2. 1.12) has classically been regarded as an ubiquitous enzyme of little importance beyond its role in glycolysis. Indeed, the most frequent reference to GAPDH in recent scientific literature is as the “housekeeping” gene used to standardize Northern blots. A careful examination of the literature of the last 25 years, however, reveals a number of novel actions of GAPDH beyond a role in glycolysis. A review of these novel functions of GAPDH is beyond the scope of this chapter, and the reader is referred to a number of recent thorough reviews by Michael Sirover for further details (1,2). Of the novel functions identified for GAPDH, one of the most intriguing is as an integral part of one or more apoptotic cascades. Here, we will briefly outline the evidence for GAPDH not only playing a role in the initiation of apoptosis, but also being a target of a number of known antiapoptotic compounds. A more in-depth discussion of the role of GAPDH in apoptosis is provided in our recent review article (3).

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Authors and Affiliations

  1. ALviva Biopharmaceuticals Inc., Saskatoon, Saskatchewan, Canada

    Mark D. Berry PHD & Paula C. Ashe PHD

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  1. Mark D. Berry PHD
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  2. Paula C. Ashe PHD
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Editors and Affiliations

  1. Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada

    Andréa C. LeBlanc

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© 2002 Humana Press Inc., Totowa, NJ

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Berry, M.D., Ashe, P.C. (2002). Glyceraldehyde-3-phosphate Dehydrogenase as a Target for Antiapoptotic Drugs. In: LeBlanc, A.C. (eds) Apoptosis Techniques and Protocols. Neuromethods, vol 37. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-188-4:149

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  • DOI: https://doi.org/10.1385/1-59259-188-4:149

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-1-58829-012-0

  • Online ISBN: 978-1-59259-188-6

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