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Monitoring Caspases in Neuronal Cell Death

  • Protocol
Apoptosis Techniques and Protocols

Part of the book series: Neuromethods ((NM,volume 37))

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Abstract

Apoptosis is a physiological process that contributes to the establishment and homeostasis of the nervous system. For example, neurons that fail to make the proper connections with their postsynaptic targets die naturally during development due to the lack of sufficient trophic support (1). Cell demise is operated in a wellordered fashion and culminates in the activation of a network of specific proteases, the caspases that execute the death program. Once activated by the apoptotic signal, caspases cleave a myriad of cellular target proteins in a highly specific fashion. As a consequence, the death signal is amplified, and the committed cell systematically dismantled (2). We describe here a practical guide to investigate the involvement of caspases in neuronal cell death.

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Authors and Affiliations

  1. Bloomfield Center for Reseach in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada

    Younes Bounhar BSc, Omar Tounekti PHD & Andréa C. LeBlanc PHD

  2. Department Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada

    Younes Bounhar BSc

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  1. Younes Bounhar BSc
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  2. Omar Tounekti PHD
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  3. Andréa C. LeBlanc PHD
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Editors and Affiliations

  1. Bloomfield Center for Research in Aging, Lady Davis Institute for Medical Research, Jewish General Hospital and Department of Neurology and Neurosurgery, McGill University, Montreal, Quebec, Canada

    Andréa C. LeBlanc

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Bounhar, Y., Tounekti, O., LeBlanc, A.C. (2002). Monitoring Caspases in Neuronal Cell Death. In: LeBlanc, A.C. (eds) Apoptosis Techniques and Protocols. Neuromethods, vol 37. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-188-4:035

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  • DOI: https://doi.org/10.1385/1-59259-188-4:035

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-1-58829-012-0

  • Online ISBN: 978-1-59259-188-6

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