Abstract
Airway surface liquid (ASL) is generally described as a two-layer system formed by a “sol” (or periciliary) and a “gel” layer (overlying the cilia). The ASL, whose total thickness is estimated to be between 10 and 40 µm, contributes to mechanical and antimicrobial defense systems in host airways. In cystic fibrosis (CF), lack of functional cystic fibrosis transmembrane conductance regulator (CFTR) protein may modulate the composition of ASL and lead to an impaired mucociliary clearance associated with inhibition of host defense activity.
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Matsui, H., Grubb, B. R., Tarran, R., Randell, S. H., Gatzy, J.T., Davis, C. W., and Boucher, R. C. (1998) Evidence for periciliary liquid layer depletion, not abnormal ion composition, in the pathogenesis of cystic fibrosis airways disease. Cell 95, 1005–1015.
Boucher, R. C., Stutts, M. J., Bromberg, P. A., and Gatzy, J. T. (1981) Regional differences in airway surface liquid composition. J. Appl. Physiol. 50 (3), 613–620.
Joris, L. and Quinton, P. M. (1987) Concentration of elements in airway surface fluid. Med. Sci. Res. 15, 855–856.
Joris, L. and Quinton, P. M. (1992) Filter paper equilibration as o novel technique for in vitro studies of the composition of airway surface fluid. Am. J. Physiol. 263, L243–L248.
Joris, L., Dab, I., and Quinton, P. M. (1993) Elemental composition of human airway surface fluid on healthy and diseased airways. Am. Rev. Respir. Dis. 148, 1633–1637.
Robinson, N. P., Kyle, H., Webber, S. E., and Widdicombe, I. G. (1989) Electrolyte and other chemical concentrations in tracheal airway surface liquid and mucus. J. itAppl. Physiol. 66(5), 2129–2135.
Govindaraju, K., Cowley, E. A., Eidelman, D. H., and Lloyd, D. K. (1997) Microanalysis of airway surface fluid by capillary electrophoresis with conductivity detection. An. Chem. 69, 2793–2797.
Baconnais, S., Zahm, J. M., Killian, L., Bonhomme, P., Gobillard, D., Perchet, A., Puchelle, E., and Balossier, G. (1998) X-ray microanalysis of native airway surface liquid collected by cryotechnique. J. Microsc. 191, 311–319.
Hall, T. A. (1979) Biological X-ray microanalysis. J. Microsc. 117, 145–163.
Hall, T. A. and Gupta, B. L. (1982) Quantification for the X-ray microanalysis of cryosections. J. itMicrosc. 126, 333–345.
Wagner, D., Puchelle, E., Hinnrasky, J., Girard, P., and Balossier, G. (1994) Quantitative X-ray microanalysis of P, Ca and S in the mucus secretory granules of cryofixed frog palate epithelium. Microsc. Res. Technol. 28, 141–148.
Zaluzec, N. J. (1989) Processing and quantification of x-ray energy dispersive spectra in the analytical electron microscope. Ultramicroscopy 28, 226–235.
Kitzawa, T., Shuman, H., and Somlyo, A.P. (1983) Quantitative electron probe analysis: problems and solutions. Ultramicroscopy 11, 251–262.
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© 2002 Humana Press Inc.
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Zahm, JM., Baconnais, S., Balossier, G., Puchelle, E. (2002). Technical Approaches to Analyze the In Vivo Ion Composition of Airway Surface Liquid. In: Skach, W.R. (eds) Cystic Fibrosis Methods and Protocols. Methods in Molecular Medicine™, vol 70. Humana Press. https://doi.org/10.1385/1-59259-187-6:563
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DOI: https://doi.org/10.1385/1-59259-187-6:563
Publisher Name: Humana Press
Print ISBN: 978-0-89603-897-4
Online ISBN: 978-1-59259-187-9
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