Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold

  • Lisa A. Catapano
  • Sanjay S. P. Magavi
  • Jeffrey D. Macklis
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 198)

Abstract

The study of neuronal connectivity requires the ability to trace axons from the neuronal cell body to its axon terminal (anteriograde tracing) and from the terminal back to the soma (retrograde tracing). Such neuroanatomical tracing is frequently used to identify neurons on the basis of their pre- or post-synaptic connections. Neuroanatomical tracing has become particularly important in the field of nervous system regeneration and repair, allowing investigators to follow the axon projections of newly born, transplanted, or axotomized neurons in lesioned or neurodegenerative environments. To allow further study of neurons identified and labeled in this way, it is particularly important that tracers are compatible with other tissue processing such as immunocytochemistry.

Keywords

Syringe Sponge Gelatin Paraformaldehyde Diaminobenzidine 

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Lisa A. Catapano
    • 1
  • Sanjay S. P. Magavi
    • 1
  • Jeffrey D. Macklis
    • 1
  1. 1.Division of Neuroscience, Children’s Hospital, Program in NeuroscienceHarvard Medical SchoolBoston

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