Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
The study of neuronal connectivity requires the ability to trace axons from the neuronal cell body to its axon terminal (anteriograde tracing) and from the terminal back to the soma (retrograde tracing). Such neuroanatomical tracing is frequently used to identify neurons on the basis of their pre- or post-synaptic connections. Neuroanatomical tracing has become particularly important in the field of nervous system regeneration and repair, allowing investigators to follow the axon projections of newly born, transplanted, or axotomized neurons in lesioned or neurodegenerative environments. To allow further study of neurons identified and labeled in this way, it is particularly important that tracers are compatible with other tissue processing such as immunocytochemistry.
KeywordsProjection Neuron Axon Terminal Neuronal Cell Body Distal Dendrite Injured Axon
- 7.Romero, M. I. and Phelps, C. J. (1997) Identification of growth hormone-releasing hormone and somatostatin neurons projecting to the median eminence in normal and growth hormone-deficient Ames dwarf mice. Neuro endocrinology 65(2), 107–116.Google Scholar
- 19.Shin, J. J., Fricker-Gates, R. A., Perez, F. A., Leavitt, B. R., Zurakowski, D.,and Macklis, J. D. (2000) Transplanted neuroblasts differentiate appropriately into projection neurons with correct neurotransmitter and receptor phenotype in neocortex undergoing targeted projection neuron degeneration. J. Neurosci. 20, 7404–7416.PubMedGoogle Scholar
- 20.Fricker-Gates, R. A., Shin, J. J., Catapano, L. A., Tai, C. C., and Macklis, J. D.,unpublished results.Google Scholar