Abstract
Studies of the rat mammary carcinogenesis model have provided useful information on the mechanisms of human breast cancer. The rat model demonstrated that chemical carcinogens are causative agents of mammary cancer and that initiation of cancer requires the interaction of the carcinogen with an undifferentiated and proliferating mammary gland. Comparative studies between humans and rodents have allowed us to determine that mammary cancer originates in undifferentiated terminal structures of the mammary gland. The terminal ducts of the Lob 1 of the human female breast have many points in common with the terminal end buds (TEB) of the rat mammary gland. Both the TEB in the rat and the Lob 1 in women are the site of origin of ductal carcinoma (1). Consequently, investigating the mechanisms involved in human breast cancer in an established rat animal model is a necessity. Various in vitro assays have been designed to understand the mechanisms of chemically induced neoplastic transformation of mammary gland epithelial cells.
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References
Russo, J. and Russo, I. H. (1993) Developmental pattern of the human breast and susceptibility to carcinogenesis. Eur. J. Cancer Prev. 3, 85ā100.
Brennan, M. J. (1976) Murine and rat mammary tumors as models for the immunological study of human breast cancer. Cancer Res. 36, 728ā733.
Dao, T. L. and Sinha, D. (1972) Mammary adenocarcinoma induced in organ culture by 7,12dimethybenzanthracene. J. Natl. Cancer Inst. 49, 591ā593.
Richards, J. and Nandi, S. (1978) Neoplastic transformation of rat mammary cells exposed to 7,12-dimethylbenzanthracene or N-nitrosomethylurea in cell culture. Proc. Natl. Acad. Sci. USA 75, 38ā68.
Greiner, J. W., Dipaolo, J. A., and Evans, C. H. (1983) Carcinogen-induced phenotypic alterations in mammary epithelial cells accompanying the development of neoplastic transformation. Cancer Res. 43, 273ā278.
Welsch, C. W. (1985) Host factors affecting the growth of carcinogen induced rat mammary carcinomas: a review and tribute to Charles Brenton Huggins. Cancer Res. 45, 3415ā3443.
Stoica, G., Koestner, A., and Capen, C. (1983) Characteristics of N-ethyl-N-nitrosourea induced mammary tumors in the rat. Am. J. Pathol. 110, 161ā169.
Stoica, G., Koestner, A., and Capen, C. (1984) Neoplasms induced with high single doses of N-ethyi-N-nitrosourea in 30-day-old Sprague-DawIey rats with special emphasis on mammary neoplasia. Anticancer Res. 4, 5ā12.
Gichner, T. and Veleminsky, J. (1982) Genetic effects of N-methyl-Nā-nitro-N-nitrosoquanidine and its homologs. Mutation Res. 99, 129ā242.
Singer, B. (1979) N-nitroso alkylating agents: formation and persistence of alkyl derivatives in mammalian nucleic acids as contributing factors in carcinogenesis. J. Natl. Cancer Inst. 6, 1329ā1339.
Druckrey, H. (1973) Chemical structure and action in transplacental carcinogenesis and teratogenesis, p. 45. In Transplacental carcinogenesis, publication no. 4. International Agency for Cancer Research, Lyon.
Magee, P. N., Barnes, J. M. (1969) Carcinogenic nitroso compounds. Adv. Cancer Res. 10, 163ā246.
Rice, J. M. (1973) An overview of transplacental chemical carcinogenesis. Teratology 8, 113ā135.
Franks, L. M. and Wigley, C, B. (1979) In vitro transformation in the respiratory tract, p. 137ā162. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Fusenig, N., Breitkreutz, D., Boukamp, P., Lueder, M., Irmscher, G., and Worst, P. K. M. (1979) Chemical carcinogenesis in mouse epidermal cultures: altered expression of tissue specific functions accompanying cell transformation, p. 37ā98. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Indo, K. and Maivaji, H. (1979) Neoplastic transformation and abnormal differentiation in foetal rat keratinizing epidermal cells in culture, p. 99ā112. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Kuroki, T., Drevon, C., Saint Vincent, L., and Montesano, R. (1979) Properties of the IAR-series of liver epithelial cells transformed by chemical carcinogens, p. 173ā188. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Stimmerhaves, I. C. (1979) Influence of donor a-c on in vitro transformation of bladder epithelium, p. 137ā162. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Wigley, C. B. (1979) Transformation in vitro of adult mouse salivary gland epithelium; a system for studies on mechanisms of initiation and promotion, p. 3ā6. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Au, W., Soukup, S. W., and Mandybur, T. I. (1977) Excess chromosome #4 in ethylnitrosourea-induced neurogenic tumor lines of the rat. J. Natl. Cancer Inst. 59, 1709ā1716.
Laerum, O. and Rajaewsky, M. E. (1975) Neoplastic transformation of fetal rat brain cells in culture after exposure to ethylnitrosourea in vivo. J. Natl. Cancer Inst. 5-5, 1177ā1187.
Stoica, G., Koestner, A., and OāLeary, M. (1985) Characteristics of normal rat mammary epithelial cells and N-ethyl-N-nitrosourea-induced adenocarcinoma cells grown in culture. Anticancer Res. 5, 499ā510.
Krishan, A. (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J. Cell Biol. 66, 188ā193.
Levan, G. and Mitleman, F. (1976) G-banding analysis in a serially transplanted Rous rat sarcoma. Hereditas 80, 140ā145.
Wooranooj, U. and Hsu, T. C. (1972) The C-and G-banding patterns of Rattus norvegicus. J. Natl. Cancer Inst. 49, 1425ā1431.
Yunis, J. J. (1981) New chromosome techniques in the study of human neoplasia. Hum. Pathol. 12, 450ā549.
Buckton, K. E. and Evans, H. J. (1973) Methods for the analysis of human chromosome aberrations. World Health Organization, 18ā22.
Commitee for a Standard Karyotype of Rattus norvegicus. (1973) Standard karyotype of the Norway rat Rattus norvegicus. Cytogenet. Cell Genet. 12, 199ā205.
Chen, T. R. (1977) In situ detection of mycoplasma contamination in cell cultures by Hoechst 33258 stain, Cell Res. 104, 255ā262.
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Stoica, G. (2002). ENU Mutagenesis of Rat Mammary Epithelial Cells. In: Wise, C. (eds) Epithelial Cell Culture Protocols. Methods In Molecular Medicineā¢, vol 188. Humana Press. https://doi.org/10.1385/1-59259-185-X:291
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DOI: https://doi.org/10.1385/1-59259-185-X:291
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