Abstract
Studies of the rat mammary carcinogenesis model have provided useful information on the mechanisms of human breast cancer. The rat model demonstrated that chemical carcinogens are causative agents of mammary cancer and that initiation of cancer requires the interaction of the carcinogen with an undifferentiated and proliferating mammary gland. Comparative studies between humans and rodents have allowed us to determine that mammary cancer originates in undifferentiated terminal structures of the mammary gland. The terminal ducts of the Lob 1 of the human female breast have many points in common with the terminal end buds (TEB) of the rat mammary gland. Both the TEB in the rat and the Lob 1 in women are the site of origin of ductal carcinoma (1). Consequently, investigating the mechanisms involved in human breast cancer in an established rat animal model is a necessity. Various in vitro assays have been designed to understand the mechanisms of chemically induced neoplastic transformation of mammary gland epithelial cells.
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Russo, J. and Russo, I. H. (1993) Developmental pattern of the human breast and susceptibility to carcinogenesis. Eur. J. Cancer Prev. 3, 85–100.
Brennan, M. J. (1976) Murine and rat mammary tumors as models for the immunological study of human breast cancer. Cancer Res. 36, 728–733.
Dao, T. L. and Sinha, D. (1972) Mammary adenocarcinoma induced in organ culture by 7,12dimethybenzanthracene. J. Natl. Cancer Inst. 49, 591–593.
Richards, J. and Nandi, S. (1978) Neoplastic transformation of rat mammary cells exposed to 7,12-dimethylbenzanthracene or N-nitrosomethylurea in cell culture. Proc. Natl. Acad. Sci. USA 75, 38–68.
Greiner, J. W., Dipaolo, J. A., and Evans, C. H. (1983) Carcinogen-induced phenotypic alterations in mammary epithelial cells accompanying the development of neoplastic transformation. Cancer Res. 43, 273–278.
Welsch, C. W. (1985) Host factors affecting the growth of carcinogen induced rat mammary carcinomas: a review and tribute to Charles Brenton Huggins. Cancer Res. 45, 3415–3443.
Stoica, G., Koestner, A., and Capen, C. (1983) Characteristics of N-ethyl-N-nitrosourea induced mammary tumors in the rat. Am. J. Pathol. 110, 161–169.
Stoica, G., Koestner, A., and Capen, C. (1984) Neoplasms induced with high single doses of N-ethyi-N-nitrosourea in 30-day-old Sprague-DawIey rats with special emphasis on mammary neoplasia. Anticancer Res. 4, 5–12.
Gichner, T. and Veleminsky, J. (1982) Genetic effects of N-methyl-N’-nitro-N-nitrosoquanidine and its homologs. Mutation Res. 99, 129–242.
Singer, B. (1979) N-nitroso alkylating agents: formation and persistence of alkyl derivatives in mammalian nucleic acids as contributing factors in carcinogenesis. J. Natl. Cancer Inst. 6, 1329–1339.
Druckrey, H. (1973) Chemical structure and action in transplacental carcinogenesis and teratogenesis, p. 45. In Transplacental carcinogenesis, publication no. 4. International Agency for Cancer Research, Lyon.
Magee, P. N., Barnes, J. M. (1969) Carcinogenic nitroso compounds. Adv. Cancer Res. 10, 163–246.
Rice, J. M. (1973) An overview of transplacental chemical carcinogenesis. Teratology 8, 113–135.
Franks, L. M. and Wigley, C, B. (1979) In vitro transformation in the respiratory tract, p. 137–162. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Fusenig, N., Breitkreutz, D., Boukamp, P., Lueder, M., Irmscher, G., and Worst, P. K. M. (1979) Chemical carcinogenesis in mouse epidermal cultures: altered expression of tissue specific functions accompanying cell transformation, p. 37–98. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Indo, K. and Maivaji, H. (1979) Neoplastic transformation and abnormal differentiation in foetal rat keratinizing epidermal cells in culture, p. 99–112. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Kuroki, T., Drevon, C., Saint Vincent, L., and Montesano, R. (1979) Properties of the IAR-series of liver epithelial cells transformed by chemical carcinogens, p. 173–188. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Stimmerhaves, I. C. (1979) Influence of donor a-c on in vitro transformation of bladder epithelium, p. 137–162. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Wigley, C. B. (1979) Transformation in vitro of adult mouse salivary gland epithelium; a system for studies on mechanisms of initiation and promotion, p. 3–6. In Neoplastic transformation in differentiated epithelial cell systems in vitro (Franks and Wigley, eds.). Academic Press, New York.
Au, W., Soukup, S. W., and Mandybur, T. I. (1977) Excess chromosome #4 in ethylnitrosourea-induced neurogenic tumor lines of the rat. J. Natl. Cancer Inst. 59, 1709–1716.
Laerum, O. and Rajaewsky, M. E. (1975) Neoplastic transformation of fetal rat brain cells in culture after exposure to ethylnitrosourea in vivo. J. Natl. Cancer Inst. 5-5, 1177–1187.
Stoica, G., Koestner, A., and O’Leary, M. (1985) Characteristics of normal rat mammary epithelial cells and N-ethyl-N-nitrosourea-induced adenocarcinoma cells grown in culture. Anticancer Res. 5, 499–510.
Krishan, A. (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J. Cell Biol. 66, 188–193.
Levan, G. and Mitleman, F. (1976) G-banding analysis in a serially transplanted Rous rat sarcoma. Hereditas 80, 140–145.
Wooranooj, U. and Hsu, T. C. (1972) The C-and G-banding patterns of Rattus norvegicus. J. Natl. Cancer Inst. 49, 1425–1431.
Yunis, J. J. (1981) New chromosome techniques in the study of human neoplasia. Hum. Pathol. 12, 450–549.
Buckton, K. E. and Evans, H. J. (1973) Methods for the analysis of human chromosome aberrations. World Health Organization, 18–22.
Commitee for a Standard Karyotype of Rattus norvegicus. (1973) Standard karyotype of the Norway rat Rattus norvegicus. Cytogenet. Cell Genet. 12, 199–205.
Chen, T. R. (1977) In situ detection of mycoplasma contamination in cell cultures by Hoechst 33258 stain, Cell Res. 104, 255–262.
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Stoica, G. (2002). ENU Mutagenesis of Rat Mammary Epithelial Cells. In: Wise, C. (eds) Epithelial Cell Culture Protocols. Methods In Molecular Medicine™, vol 188. Humana Press. https://doi.org/10.1385/1-59259-185-X:291
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DOI: https://doi.org/10.1385/1-59259-185-X:291
Publisher Name: Humana Press
Print ISBN: 978-0-89603-893-6
Online ISBN: 978-1-59259-185-5
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