Assessing Epithelial Cell Confluence by Spectroscopy
There is an increasing use of epithelial cells grown on tissue culture supports. Such tissue culture systems are used to study the cellular mechanisms involved in protein and lipid sorting between the two series membranes, which comprise the polarized epithelia cell, the molecular biology of transporter assembly, and the study of transporter associated proteins. By necessity, these studies start after the tissue culture cells reach confluence and form tight junctions.
KeywordsTissue Culture Medium Tissue Culture Cell Tissue Culture System Plasma Compartment Plasma Solution
- 2.Wills, N. K. (1996) Epithelial cell culture, p. 236–255. In Epithelial transport: a guide to methods and experimental analysis. (Wills, N. K., Reuss, L., and Lewis, S. A., eds.). Chapman and Hall, London.Google Scholar
- 3.Delamere, N. A. and Duncan, G. (1977) A comparison of ion concentrations, potentials and conductances of amphibian, bovine and cephalod lenses. J. Physiol. 271, 167–186.Google Scholar