Assessing Epithelial Cell Confluence by Spectroscopy

  • Simon A. Lewis
Protocol
Part of the Methods In Molecular Medicine™ book series (MIMB, volume 188)

Abstract

There is an increasing use of epithelial cells grown on tissue culture supports. Such tissue culture systems are used to study the cellular mechanisms involved in protein and lipid sorting between the two series membranes, which comprise the polarized epithelia cell, the molecular biology of transporter assembly, and the study of transporter associated proteins. By necessity, these studies start after the tissue culture cells reach confluence and form tight junctions.

Keywords

Permeability Quartz Phenol Penicillin CaCl2 

References

  1. 1.
    Jovov, B., Wills, N. K., and Lewis, S. A. (1991) A spectroscopic method for assessing confluence of epithelial cell culture. Am. J. Physiol. 261, C1196–C1203.PubMedGoogle Scholar
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    Wills, N. K. (1996) Epithelial cell culture, p. 236–255. In Epithelial transport: a guide to methods and experimental analysis. (Wills, N. K., Reuss, L., and Lewis, S. A., eds.). Chapman and Hall, London.Google Scholar
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    Delamere, N. A. and Duncan, G. (1977) A comparison of ion concentrations, potentials and conductances of amphibian, bovine and cephalod lenses. J. Physiol. 271, 167–186.Google Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Simon A. Lewis
    • 1
  1. 1.Department of Physiology and BiophysicsUniversity of Texas Medical BranchGalveston

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