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The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains

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Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 173))

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Abstract

The calcium-binding (cb) epidermal growth factor-like (EGF) domain is a common variant of the EGF module that contains a consensus sequence associated with ligation of a single calcium ion (1-3). Many extracellular proteins include cbEGF domains, and several of these have been associated with human disease (reviewed in ref. 4). Two examples from this group are human fibrillin-1 and the low-density liproprotein (LDL) receptor. Moreover, mutations within these proteins have been linked to the Marfan syndrome and familial hypercholesterolaemia, respectively. Recently, we have been using solution NMR to probe the structure, calcium-binding properties and dynamics of tandem cbEGF domains from these two proteins.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and OCMS, University of Oxford, South Parks Road, Oxford, UK

    Jonathan Boyd, Iain D. Campbell & Kristina Downing A.

Authors
  1. Jonathan Boyd
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  2. Iain D. Campbell
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  3. Kristina Downing A.
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Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary Calgary, AB, Canada

    Hans J. Vogel

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© 2002 Humana Press Inc.

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Boyd, J., Campbell, I.D., A., K.D. (2002). The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains. In: Vogel, H.J. (eds) Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques. Methods in Molecular Biology™, vol 173. Springer, Totowa, NJ. https://doi.org/10.1385/1-59259-184-1:301

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  • DOI: https://doi.org/10.1385/1-59259-184-1:301

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-689-5

  • Online ISBN: 978-1-59259-184-8

  • eBook Packages: Springer Protocols

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