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Crystallization and Structure-Function of Calsequestrin

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Calcium-Binding Protein Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 172))

Abstract

X-ray crystallography provides one of the most important tools for understanding structure-function relationships in proteins. Over the last several years, the scientific community has accumulated a great deal of experience making crystals and solving protein structures by X-ray diffraction. However, often the X-ray approach cannot be used because of the failure to obtain crystals, which is still considered as the single greatest experimental problem in crystallography. Currently, there is no method for predicting the conditions needed to obtain crystals for a given protein. Researchers simply screen a large number of conditions hoping to find crystals in any of the trials. Given crystals under one set of conditions, even crystals of low quality, conditions in the neighborhood of the successful trial are again surveyed in hopes of finding conditions for obtaining improved crystals. Even though crystals are obtained, some are not suitable for structure determination by X-ray diffraction. The first requirement is that a crystal must contain a sufficiently high degree of order relating one molecule to another over a sufficient number of molecules. In addition, a crystal must have an appropriate size and shape. Needle-like crystals are of insufficient size in two dimensions and may require application of seeding techniques using initial crystals as nuclei for larger crystals, but it is often not a straightforward procedure.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Washington State University, Pullman, Washington

    ChulHee Kang & A. Keith. Dunker

  2. Agricultural Experiment Station, University of New Hampshire, Durham, New Hampshire

    William R. Trumble

Authors
  1. ChulHee Kang
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  2. William R. Trumble
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  3. A. Keith. Dunker
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Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Calgary, Calgary, AB, Canada

    Hans J. Vogel

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© 2002 Humana Press Inc.

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Kang, C., Trumble, W.R., Dunker, A.K. (2002). Crystallization and Structure-Function of Calsequestrin. In: Vogel, H.J. (eds) Calcium-Binding Protein Protocols. Methods in Molecular Biology™, vol 172. Humana Press. https://doi.org/10.1385/1-59259-183-3:281

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  • DOI: https://doi.org/10.1385/1-59259-183-3:281

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-688-8

  • Online ISBN: 978-1-59259-183-1

  • eBook Packages: Springer Protocols

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