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Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB)

  • Rolf Nuck
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 194)

Abstract

When performing a structural analysis of N-glycans, a number of aspects should be considered. N-Glycans may be hydrolyzed from purified glycoproteins, serum glycoprotein mixtures, or delipidated membrane fractions by chemical hydrolysis using hydrazine or enzymatical hydrolysis using PNGase F.

Keywords

Sialic Acid Vacuum Centrifuge Neuraminic Acid Sodium Cyanoborohydride Fume Cupboard 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Rolf Nuck
    • 1
  1. 1.Institut für Molekularbiologie und BiochemieFreie Universität BerlinBerlinGermany

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