Enzymatical Hydrolysis of N-Glycans from Glycoproteins and Fluorescent Labeling by 2-Aminobenzamide (2-AB)

  • Rolf Nuck
Part of the Methods in Molecular Biology™ book series (MIMB, volume 194)


When performing a structural analysis of N-glycans, a number of aspects should be considered. N-Glycans may be hydrolyzed from purified glycoproteins, serum glycoprotein mixtures, or delipidated membrane fractions by chemical hydrolysis using hydrazine or enzymatical hydrolysis using PNGase F.


Sialic Acid Vacuum Centrifuge Neuraminic Acid Sodium Cyanoborohydride Fume Cupboard 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Rolf Nuck
    • 1
  1. 1.Institut für Molekularbiologie und BiochemieFreie Universität BerlinBerlinGermany

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