Homogeneous Techniques for Monitoring Receptor–Ligand Interactions
Radioligand binding is a well-established technique that has been used to study the interactions of extracellular ligands and their corresponding cell-surface receptors (1). The initial studies relied on laborious, vacuum-filtration techniques that are cumbersome and difficult to automate. Despite these limitations, filtration-based radioligand binding assays were adequate to probe the kinetics of receptor-ligand interactions and to screen small libraries of compounds in search of receptor-binding antagonists. A number of reviews are available that described this methodology in detail (2,3).
KeywordsWheat Germ Agglutinin Vacuum Filtration Fluorescence Correlation Spectroscopy Assay Volume Scintillation Proximity Assay
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