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Homogeneous Techniques for Monitoring Receptor–Ligand Interactions

  • James S. Marks
  • Douglas S. Burdette
  • David A. Giegel
Part of the Methods in Molecular Biology™ book series (MIMB, volume 190)

Abstract

Radioligand binding is a well-established technique that has been used to study the interactions of extracellular ligands and their corresponding cell-surface receptors (1). The initial studies relied on laborious, vacuum-filtration techniques that are cumbersome and difficult to automate. Despite these limitations, filtration-based radioligand binding assays were adequate to probe the kinetics of receptor-ligand interactions and to screen small libraries of compounds in search of receptor-binding antagonists. A number of reviews are available that described this methodology in detail (2,3).

Keywords

Wheat Germ Agglutinin Vacuum Filtration Fluorescence Correlation Spectroscopy Assay Volume Scintillation Proximity Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • James S. Marks
    • 1
  • Douglas S. Burdette
    • 1
  • David A. Giegel
    • 1
  1. 1.Pfizer Global Research and DevelopmentAnn Arbor

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