Homogeneous Techniques for Monitoring Receptor–Ligand Interactions
Radioligand binding is a well-established technique that has been used to study the interactions of extracellular ligands and their corresponding cell-surface receptors (1). The initial studies relied on laborious, vacuum-filtration techniques that are cumbersome and difficult to automate. Despite these limitations, filtration-based radioligand binding assays were adequate to probe the kinetics of receptor-ligand interactions and to screen small libraries of compounds in search of receptor-binding antagonists. A number of reviews are available that described this methodology in detail (2,3).
KeywordsSucrose Filtration Albumin Carbohydrate EDTA
- 2.Bylund D. B. and Yamamura H. I. (1990) Methods for receptor binding, in Methods in Neurotransmitter Receptor Analysis (Yamamura H. I., Enna S. J., and Kuha M. J., (nteds.), Raven Press, New York, NY pp. 1–35.Google Scholar
- 3.Qume M. (1999) Overview of ligand-receptor binding techniques, in Methods in Molecular Biology (Keen M., ed.), Humana Press Inc., Totowa, NJ, 3–23.Google Scholar
- 8.Haupts U., Rudiger M., and Pope A. J. (2000) Macroscopic versus microscopic fluorescence techniques in (ultra)-high throughput screening. DDT:HTS (Suppl. 1), 3–9Google Scholar
- 9.Atkins P. W. (1994) Physical chemistry, 5th ed. W. H. Freeman Co., New York, NY, pp. 1–17.Google Scholar