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In Situ Detection of DNA Damage pp 31–39Cite as

Electron Microscopic Detection of DNA Damage Labeled by TUNEL

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 203))

Abstract

A number of exogenous and endogenous toxic agents may damage DNA, leading to genomic instability and transcriptional infidelity. Genetic or acquired defects in DNA repair mechanisms also contribute to exacerbate DNA damage. Progressive accumulation of DNA injury may alter the genetic control of cell proliferation and cause cancer. Alternatively, increased cell death may be a more likely consequence if DNA damage affects postmitotic cells, e.g., neurons (1). As a matter of fact, DNA damage is suspected to play a major role in the neuronal death that characterizes brain ischemia (2) and various neurodegenerative diseases (1,3).

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Author information

Authors and Affiliations

  1. Laboratory of Neuropathology, Department of Neuroscience, University of Turin, Turin, Italy

    Antonio Migheli

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  1. Antonio Migheli
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Editor information

Editors and Affiliations

  1. Baylor College of Medicine, Houston, TX

    Vladimir V. Didenko

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© 2002 Humana Press Inc.

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Migheli, A. (2002). Electron Microscopic Detection of DNA Damage Labeled by TUNEL. In: Didenko, V.V. (eds) In Situ Detection of DNA Damage. Methods in Molecular Biology, vol 203. Humana Press. https://doi.org/10.1385/1-59259-179-5:31

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  • DOI: https://doi.org/10.1385/1-59259-179-5:31

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-952-0

  • Online ISBN: 978-1-59259-179-4

  • eBook Packages: Springer Protocols

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