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In Situ Detection of Double-Strand DNA Breaks with Terminal 5′OH Groups

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In Situ Detection of DNA Damage

Part of the book series: Methods in Molecular Biology ((MIMB,volume 203))

Abstract

DNA fragmentation is often used as a marker of programmed cell death in tissue sections. It is a sensitive indicator of apoptosis, particularly when utilized in combination with morphological verification. At least two types of DNA breaks are present in apoptotic cells: 1. Breaks with 3′OH and 5′PO4 groups at the ends of the break, which can be generated by DNase I and DNase I-like nucleases; 2. Breaks with the inverted distribution of these chemical groups—3′ PO4/5′OH, produced by DNase II and DNase II-like nucleases (1-4).

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Author information

Authors and Affiliations

  1. Molecular and Cell Biology Laboratory, Department of Neurosurgery, Baylor College of Medicine, Houston, TX

    Vladimir V. Didenko & Hop Ngo

  2. Department of Neurosurgery, Baylor College of Medicine, Houston, TX

    David S. Baskin

Authors
  1. Vladimir V. Didenko
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  2. Hop Ngo
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  3. David S. Baskin
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Editor information

Editors and Affiliations

  1. Baylor College of Medicine, Houston, TX

    Vladimir V. Didenko

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© 2002 Humana Press Inc.

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Didenko, V.V., Ngo, H., Baskin, D.S. (2002). In Situ Detection of Double-Strand DNA Breaks with Terminal 5′OH Groups. In: Didenko, V.V. (eds) In Situ Detection of DNA Damage. Methods in Molecular Biology, vol 203. Humana Press. https://doi.org/10.1385/1-59259-179-5:153

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  • DOI: https://doi.org/10.1385/1-59259-179-5:153

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-952-0

  • Online ISBN: 978-1-59259-179-4

  • eBook Packages: Springer Protocols

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