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PCR-Based Screening for Bacterial Artificial Chromosome Libraries

  • Yuji Yasukochi
Part of the Methods in Molecular Biology™ book series (MIMB, volume 192)

Abstract

The bacterial artificial chromosome (BAC) (1) is now the most widely utilized vector system to construct large-insert genomic libraries for genome analysis. It has the following advantages over another vector system, the yeast artificial chromosome (YAC) (2): ease of DNA purification, a lower rate of chimerism, stable maintenance of inserts, and compatibility with templates for sequence determination. Regarding screening, it is very important that BAC-DNAs can be separated from genomic DNA of the host, as the host DNA often competes with BAC-DNAs for the amplification of expected PCR fragments.

Keywords

Bacterial Artificial Chromosome Positive Clone Bacterial Artificial Chromosome Library Yeast Artificial Chromosome Colony Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Yuji Yasukochi
    • 1
  1. 1.National Institute of Agrobiological SciencesIbarakiJapan

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