PCR-Based Screening for Bacterial Artificial Chromosome Libraries

  • Yuji Yasukochi
Part of the Methods in Molecular Biology™ book series (MIMB, volume 192)


The bacterial artificial chromosome (BAC) (1) is now the most widely utilized vector system to construct large-insert genomic libraries for genome analysis. It has the following advantages over another vector system, the yeast artificial chromosome (YAC) (2): ease of DNA purification, a lower rate of chimerism, stable maintenance of inserts, and compatibility with templates for sequence determination. Regarding screening, it is very important that BAC-DNAs can be separated from genomic DNA of the host, as the host DNA often competes with BAC-DNAs for the amplification of expected PCR fragments.


Bacterial Artificial Chromosome Positive Clone Bacterial Artificial Chromosome Library Yeast Artificial Chromosome Colony Hybridization 
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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Yuji Yasukochi
    • 1
  1. 1.National Institute of Agrobiological SciencesIbarakiJapan

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