PCR-Based Screening for Bacterial Artificial Chromosome Libraries
The bacterial artificial chromosome (BAC) (1) is now the most widely utilized vector system to construct large-insert genomic libraries for genome analysis. It has the following advantages over another vector system, the yeast artificial chromosome (YAC) (2): ease of DNA purification, a lower rate of chimerism, stable maintenance of inserts, and compatibility with templates for sequence determination. Regarding screening, it is very important that BAC-DNAs can be separated from genomic DNA of the host, as the host DNA often competes with BAC-DNAs for the amplification of expected PCR fragments.
KeywordsBacterial Artificial Chromosome Positive Clone Bacterial Artificial Chromosome Library Yeast Artificial Chromosome Colony Hybridization
- 6.Wu, C., Asakawa, S., Shimizu, N., Kawasaki, S., and Yasukochi, Y. (1999) Construction and characterization of bacterial artificial chromosome libraries from the silkworm, Bombyx mori. Mol. Gen. Genet. 261, 698–706.Google Scholar