Abstract
Heat will separate or "e;melt"e; double-stranded DNA into single-stranded DNA by disrupting its hydrogen bonds. T m (melting temperature) is the temperature at which half the DNA strands are single-stranded and half are double-stranded. T m characterizes the stability of the DNA hybrid formed between an oligonucleotide and its complementary strand and therefore is a core parameter in primer design. It is affected by primer length, primer sequence, salt concentration, primer concentration, and the presence of denaturants (such as formamide or DMSO).
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© 2002 Humana Press Inc.
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Chen, BY., Janes, H.W., Chen, S. (2002). Computer Programs for PCR Primer Design and Analysis. In: Chen, BY., Janes, H.W. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 192. Humana Press. https://doi.org/10.1385/1-59259-177-9:019
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DOI: https://doi.org/10.1385/1-59259-177-9:019
Publisher Name: Humana Press
Print ISBN: 978-0-89603-969-8
Online ISBN: 978-1-59259-177-0
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