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XL PCR Amplification of Long Targets from Genomic DNA

  • Lori A. Kolmodin
Part of the Methods in Molecular Biology™ book series (MIMB, volume 192)

Abstract

Long polymerase chain reaction (PCR)>(1 5), specifically, XL PCR (Extra-Long Polymerase Chain Reaction), has enabled amplification of expanded trinucleotide repeats of the neuromuscular disease myotonic dystrophy (6) a 9-kb HIV-1 provirus from primary isolate DNA (7), 24.2-kb fragments from nanogram quantities of genomic DNA for DNA damage repair (8), and up to 42 kb of human genomic DNA (4). The capability of the long PCR process stems through the use of:

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Amplification Standard Polymerase Chain Reaction GeneAmp Polymerase Chain Reaction System Ethidium Bromide Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Lori A. Kolmodin
    • 1
  1. 1.Roche Molecular SystemsPleasanton

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