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Oxidative Stress Biomarkers and Antioxidant Protocols pp 77–88Cite as

Detection of Certain Peroxynitrite-Induced DNA Modifications

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 186))

Abstract

Nitric oxide and superoxide rapidly combine to form a toxic reaction product, peroxynitrite anion (ONOO−) (1,2). The oxidant reactivity of peroxynitrite is mediated by an intermediate with the biological activity of the hydroxyl radical. However, this product does not appear to be the hydroxyl radical per se, but peroxynitrous acid (ONOOH) or its activated isomer (ONOOH*) (2). Peroxynitrite readily reacts with proteins, lipids, and DNA under conditions of inflammation.

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Author information

Authors and Affiliations

  1. Unit of Endogenous Cancer Risk Factors, International Agency for Research on Cancer, Lyon, France

    Hiroshi Ohshima, Vladimir Yermilov, Brigitte Pignatelli & Mitsuharu Masuda

  2. Department of Medical Chemistry, Faculty of Medicine, University of Debrecen, Debrecen, Hungary

    László. Virág

  3. Departments of Biochemistry and Biophysics, Stokes Research Institute, Children’s Hospital of Pennsylvania, The University of Pennsylvania Medical Center, Philadelphia, PA

    Jose Souza

  4. Inotek Corporation, Beverly, MA

    Csaba Szabó

Authors
  1. Hiroshi Ohshima
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  2. László. Virág
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  3. Jose Souza
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  4. Vladimir Yermilov
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  5. Brigitte Pignatelli
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  6. Mitsuharu Masuda
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  7. Csaba Szabó
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Editor information

Editors and Affiliations

  1. University of Florida, Gainesville, FL

    Donald Armstrong

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© 2002 Humana Press Inc.

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Cite this protocol

Ohshima, H. et al. (2002). Detection of Certain Peroxynitrite-Induced DNA Modifications. In: Armstrong, D. (eds) Oxidative Stress Biomarkers and Antioxidant Protocols. Methods in Molecular Biology™, vol 186. Humana Press. https://doi.org/10.1385/1-59259-173-6:77

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  • DOI: https://doi.org/10.1385/1-59259-173-6:77

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-850-9

  • Online ISBN: 978-1-59259-173-2

  • eBook Packages: Springer Protocols

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