Abstract
Lipid peroxidation is initialed as activated oxygen reacts with the double bonds on the lipid hydrocarbon chains (1). Depending on the type of lipid, type of oxidant, and severity of the oxidation, a variety of lipid-peroxidation products are formed (1). The major proucts of lipid peroxidation are moieties containing hydroxyls, hydroperoxyls, aldehydes, ketones, caroxylic acids, and trans double bonds. Infrared spectroscopy is a sensitive technique that can detect all of these groups and is uniquely sensitive in detecting hydroxyl and hydroperoxyl groups (2–8). The detection of lipid hydoxyl and hydroperoxyl groups is especially useful for quantifying the oxidation of mono unsaturated lipids, such as those found in the ocular lens, where secondary products of lipid oxidation such as malondialdeyde are not readily formed (9,11).
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References
Vigo-Pelfrey, C. (ed.) (1989) Membrane Lipid Oxidation (vols. 1-3) CRC Press, Boca Raton, FL.
Privett, O. S., Lundbergm, W. O., Khan, N. A., Tolberg, W. E., and Wheeler, D. H. (1993) Structure of hydroperoxides obtained from autoxidized methyl linoleate. J. Am. Oil Chem. Soc. 30, 61–66.
Ismail, A. A., van de Voort, F. R., Emo, G., and Sedmanm, J. (1993) Rapid quantitative determination of free fatty acids in fats and oils by Fourier transform infrared spectroscopy. J. Am. Oil Chem. Soc. 70, 335–341.
van de Voort, F. R., Ismail, A. A., Sedman, J., and Emo, G. (1994) Monitoring the oxidation of edible oils by Fourier transform infrared spectroscopy. J. Am. Oil Chem. Soc. 71, 243–253.
Dugan, L. R., Beadle, B. W., and Henick, A. S. (1949) An infrared absorption study of autoxidized methyl linoteate. J. Am. Oil Chem. Soc. 26, 681–685.
Andrews, J. S. and Leonard-Martun, T. (1981) Total lipid and membrane lipid analysis of normal animal and human lenses. Invest. Ophthalmol. 21, 39–415.
van de Voort, F. R., Sedman, J., Emo, G., and Ismail, A. A. (1992) A rapid FTIR quality control method for fat and moisture determination in butter. Food Res. Int. 25, 193–198.
van de Voort, F. R., Sedman, J., Emo, G., and Ismail, A. A. (1992) Rapid and direct iodine value and saponification number determination of fats and oils by attenuated total reflectance/fourier infrared spectroscopy. J. Am. Oil Chem. Soc. 69, 61–66.
Borchman, D. and Yappert, M. C. (1998) Age related lipid oxidation in human lenses. Invest. Ophthalmol. Vis. Sci. 39, 1053–1058.
Ahuja, R. P., Borchman, D., Dean, W. L., and Paterson, C. A., Zeng, J., Zhang, S., Ferguson-Yankey, S., and Yappert, M. C. (1999) Ca2+-ATPase kinetics and membrane oxidation relationships in lens epithelial microsomes. Free Rad. Biol. Med. 27, 177–185.
Borchman, D., Gibblin, F. J., Yappert, M. C., Leverenz, V. R., Reddy, V. N., Lin, L., Tang, D. (2000) Impact of aging and hyperbaric oxygen in vivo on guinea pig lens lipid and nuclear light scatter. Invest. Opthalmol. Vis. Sci. 41, 3061–3073.
Lamba, O. P., Borchman, D., and O’Brien, P. J. (1994) Fourier transform infrared study of the rod outer segment disk and plasma membranes of vertebrate retina. Biochemistry 33, 1704–1712.
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Borchman, D., Sinha, S. (2002). Determination of Products of Lipid Oxidation by Infrared Spectroscopy. In: Armstrong, D. (eds) Oxidative Stress Biomarkers and Antioxidant Protocols. Methods in Molecular Biology™, vol 186. Humana Press. https://doi.org/10.1385/1-59259-173-6:21
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DOI: https://doi.org/10.1385/1-59259-173-6:21
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