Abstract
Studies in many areas of biology are hampered by the complexity of the system being studied. This suggests that such areas of study could benefit from the development and application of new and more powerful analytical tools. Traditionally, investigators have chosen analytical methods that offer sensitivity and specificity, ultimately fitting collected results into an overall global picture or an evolving theory. This approach has been effective for the study of disorders manifested by a single etiology or disorders where prior knowledge indicates a clear or probable mechanism. The approach has proven less satisfying, however, when applied to complex systems with many unknown influences. Factors such as the time and cost involved in sorting through myriad possibilities as well as biological constraints (e.g., sample-size constraints) have limited analysis carried out in such complex systems. A related, although distinct, problem lies in the inability to simultaneously measure a series of related metabolites even after the relevance of the metabolic system has been identified.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Matson, W. R., Langiais, P., Volicer, L., Gamache, P. H., Bird, E. D., and Mark, K. A. (1984) n-electrode three dimensional liquid chromatography with electrochemical detection for determination of neurotransmitters. Clin. Chem 30, 1477–1488.
Svendsen, C. N. (1993) Multi-electrode array detectors in high-performance liquid chromatography: a new dimension in electrochemical analysis. Analyst 118, 123–129.
Acworth, I. N. and Gamache, P. H. (1996) The coulometric electrode array for use in HPLC analysis, Part 1. Theory. Am. Lab. 5, 33–38.
Acworth, I. N., Boweus, M. (1997) An introduction to HPLC-based electrochemical detection: From single-electrode to multi-electrode arrays. In: Coulometric Electrode Array Detections for HPLC, Progress in HPLC-HPCE, (Acworth, I.N., Naoi, M., Parvez, S., and Parvez, H., eds.) VSP Publications, Utrecht, Holland, vol. 6, pp. 1–48.
Milbury, P. E. (1997) CEAS generation of large multiparameter databases for determining categorical process involvement of biomolecules, in: Progress in HPLC, VSP International Science Publication, Utrecht, Holland. Coulometric Electrode Array Detectors for HPLC, vol. 6, pp. 125–141.
Milbury, P. E., Vaughan, M. R., Farley, S., Matula, G. J., Jr., Convertino, V. A., and Matson, W. R. A comparative bear model for immobility osteopenia. Ursus 10, 507–520.
Kristal, B. S., Vigneau-Callahan, K. E., and Matson, W. R. (1998) Simultaneous analysis of the majority of low-molecular weight, redox-active compounds from mitochondria. Anal. Biochem. 263, 18–25.
Kristal, B. S., Vigneau-Callahan, K. E., and Matson, W. R. (1999) Purine Catabolism: Links to mitochondrial respiration and antioxidant defenses? Arch. Biochem. Biophys. 370, 22–33.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc.
About this protocol
Cite this protocol
Kristal, B.S., Vigneau-Callahan, a., Matson, W.R. (2002). Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices. In: Armstrong, D. (eds) Oxidative Stress Biomarkers and Antioxidant Protocols. Methods in Molecular Biology™, vol 186. Humana Press. https://doi.org/10.1385/1-59259-173-6:185
Download citation
DOI: https://doi.org/10.1385/1-59259-173-6:185
Publisher Name: Humana Press
Print ISBN: 978-0-89603-850-9
Online ISBN: 978-1-59259-173-2
eBook Packages: Springer Protocols