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Human Xanthine Oxidoreductase Determination by a Competitive ELISA

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Oxidative Stress Biomarkers and Antioxidant Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 186))

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Abstract

Xanthine oxidoreductase (XO) catalyses the oxidation of hypoxanthine to xanthine and of the latter to uric acid. The enzyme is present in traces in most of human tissues, including plasma, being more abundant in milk, liver, and intestine (1).

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References

  1. Linder, N., Rapola, J., and Raivio, K. O. (1999) Cellular expression of xanthine oxidoreductase protein in normal human tissues Lab. Invest. 79, 967–974.

    CAS  PubMed  Google Scholar 

  2. Battelli, M. G., Abbondanza, A., Musiani, S., Buonamici, L., Strocchi, P., Tazzari, P. L., et al. (1999) Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA). Clin. Chim. Acta 281, 147–158.

    Article  CAS  PubMed  Google Scholar 

  3. Battelli, M. G., Abbondanza, A., and Stirpe, F. (1992) Effects of hypoxia and ethanol on the xanthine oxidase of isolated hepatocytes: conversion from D to O form and leakage from cells. Chem. Biol. Interactions 83, 73–84.

    Article  CAS  Google Scholar 

  4. deGroot, H. and Littauer, A. (1989) Hypoxia, reactive oxygen, and cell injury. Free Rad. Biol. Med. 6, 541–551.

    Article  PubMed  Google Scholar 

  5. Weinbroum, A. A., Hochhauser, E., Rudick, V., Kluger, Y., Karchevsky, E., Graf, E., and Vidne, B. A. (1999) Multiple organ dysfunction after remote circulatory arrest: common pathway of radical oxygen species? J. Trauma 47, 691–698.

    Article  CAS  PubMed  Google Scholar 

  6. Battelli, M. G., Buonamici, L., Polito, L., Bolognesi, A., and Stirpe, F. (1996) Hepatotoxicity of ricin, saporin or a saporin immunotoxin: xanthine oxidase activity in rat liver and blood serum. Virchows Arch. 427, 529–535.

    Article  CAS  PubMed  Google Scholar 

  7. Yamamoto, T., Moriwaki, Y., Takahashi, S., Tsutsumi, Z., Yamakita, J., Nasako, Y., et al. (1996) Determination of human plasma xanthine oxidase activity by high-performance liquid chromatography. J. Chromatogr. B. Biomed. Appl. 681, 395–400.

    Article  CAS  PubMed  Google Scholar 

  8. Pesonen, E. J., Linder, N., Raivio, K. O., Sarnesto, A., Lapatto, R., Hockerstedt, K., et al. (1998) Circulating xanthine oxidase and neutrophil activation during human liver transplantation. Gastroenterology 114, 1009–1015.

    Article  CAS  PubMed  Google Scholar 

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© 2002 Humana Press Inc.

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Battelli, M.G., Musiani, S. (2002). Human Xanthine Oxidoreductase Determination by a Competitive ELISA. In: Armstrong, D. (eds) Oxidative Stress Biomarkers and Antioxidant Protocols. Methods in Molecular Biology™, vol 186. Humana Press. https://doi.org/10.1385/1-59259-173-6:03

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  • DOI: https://doi.org/10.1385/1-59259-173-6:03

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-850-9

  • Online ISBN: 978-1-59259-173-2

  • eBook Packages: Springer Protocols

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