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Measurement of α-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry

  • Elizabeth J. Parks
Part of the Methods in Molecular Biology™ book series (MIMB, volume 186)

Abstract

As the primary fat-soluble antioxidant in human tissues, α-tocopherol absorption and metabolism have been the focus of active investigation, as reviewed recently (1,2). Although the concentration of any metabolite measured in plasma or tissue at a given time can be used as an indicator of the status of that pool, the true biological activity of molecules is better represented by their turnover. The use of stable isotopes in biological research has expanded steadily since the 1950s. The availability of methods has also increased as a result of improvements in instrument sensitivity and automation. Vitamin E studies utilizing gas chromatography-mass spectrometry (GC-MS) have aided in the elucidation of vitamin E absorption and transport, as well as the identification of vitamin E oxidation byproducts (3). Human vitamin E studies using stable isotopes of α-tocopherol (deuterated molecules) have evaluated plasma α-tocopherol levels in response to increasing vitamin E doses (4) and the selective secretion of RRR-α-tocopherol from the liver out in nascent very low-density lipoproteins (VLDL) (5,6).

Keywords

Stable Isotope Lipoprotein Fraction Tocopheryl Acetate Stoppered Bottle Standard Curve Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Traber, M. G. (1999) Vitamin E, in Modern Nutrition in Health and Disease (Shils, M. E., Olson, J. A., Shike, M., Ross, and A. C., eds.), Williams and Wilkins, Baltimore, MD, pp. 347–362.Google Scholar
  2. 2.
    Pryor, W. A. (2000) Vitamin E and heart disease: basic science to clinical intervention trials. Free Rad. Biol. Med. 28, 141–164.CrossRefPubMedGoogle Scholar
  3. 3.
    Burton, G. W., Ingold, T. U., Cheeseman, K. H., and Slater, T. F. (1990) Application of deuterated α-tocopherols to the biokinetics and bioavailability of vitamin E. Free Rad. Res. Commun. 11, 99–107.CrossRefGoogle Scholar
  4. 4.
    Traber, M. G., Tader, D., Acuff, R. V., Ramakrishnan, R., Brewer, H. B., and Kayden, H. J. (1998) Vitamin E dose-response studies in humans with use of deuterated RRR-α-tocopherol. Am. J. Clin. Nutr. 68, 847–853.PubMedGoogle Scholar
  5. 5.
    Traber, M. G., Burton, G. W., Hughes, L., et al. (1992) Discrimination between forms of vitamin E by humans with and without genetic abnormalities of lipoprotein metabolism. J. LipidRes. 33, 1171–1182.Google Scholar
  6. 6.
    Traber, M. G., Rudel, L. L., Burton, G. W., Hughes, L., Ingold, K. U., and Kayden, H. J. (1990) Nascent VLDL from liver perfusions of cynomologus monkeys are preferentially enriched in RRR- compared with SRR-α-tocopherol: studies using deuterated tocopherols. J. Lipid Res. 31, 687–694.PubMedGoogle Scholar
  7. 7.
    Kramer, J. K., Blais, L., Fouchard, R. C., Melnyk, R. A., and Kallury, K. M. (1997) A rapid method for the determination of vitamin E forms in tissues and diet by high-performance liquid chromatography using a normal-phase diol column. Lipids 32, 323–330.CrossRefPubMedGoogle Scholar
  8. 8.
    Kramer, J. K. G., Fouchard, R. C., and Kallury, K. M. R. (1999) Determination of vitamin E forms in tissues and diets by high-performance liquid chromatography using normal-phase diol column, in Methods in Enzymology, Oxidants and Antioxidants, Part A (Packer, L., ed.), Academic Press, San Diego, pp. 318–329.CrossRefGoogle Scholar
  9. 9.
    Bieri, G., Toliver, T. J., and Catignani, G. L. (1979) Simultaneous determination of α-tocopherol and retinol in plasma and red cells by high pressure liquid chromatography. Am. J. Clin. Nutr. 32, 2143–2149.PubMedGoogle Scholar
  10. 10.
    Podda, M., Weber, C., Traber, M. G., and Packer, L. (1996) Simultaneous determination of tissue tocopherols, tocotrienols, ubiquinols, and ubiquinones. J. Lipid Res. 37, 893–901.PubMedGoogle Scholar
  11. 11.
    Podda, M., Weber, C., Traber, M., Milbradt, R., and Packer, L. (1999) Sensitive high-performance liquid chromatography techniques for simultaneous determination of tocopherols, tocotrienols, ubiquinols, and ubiquinones in biological samples, in Methods in Enzymology, Oxidants and Antioxidants, Part A (Packer, L., ed.), Academic Press, San Diego, pp. 330–341.CrossRefGoogle Scholar
  12. 12.
    Rentel, C., Strohschein, S., Albert, K., and Bayer, E. (1998) Silver-plated vitamins: a method of detecting tocopherols and carotenoids in LC/ESI-MS coupling. Anal. Chem. 70, 4394–4400.CrossRefPubMedGoogle Scholar
  13. 13.
    Lauridsen, C., Leonard, S. W., Griffin, D. A. et al. (2001) Quantitative analysis by liquid chromatography-tandem mass spectrometry of deuterium-labeled and unlabeled vitamin E in biological samples. Analytical Biochemistry. 289, 89–95.CrossRefPubMedGoogle Scholar
  14. 14.
    Liebler, D. C., Burr, J. A., Philips, L., and Ham, A. J. (1996) Gas chromatographymass spectrometry analysis of vitamin E and its oxidation products. Anal. Biochem. 236, 27–34.CrossRefPubMedGoogle Scholar
  15. 15.
    Liebler, D. C., Burr, J. A., Philips, L., and Ham, A. J. L. (1999) Gas chromatography-mass spectrometry analysis of vitamin E and its oxidation products, in Methods in Enzymology, Oxidants and Antioxidants, Part A (Packer, L., ed.), Academic Press, San Diego, pp. 309–319.CrossRefGoogle Scholar
  16. 16.
    Burton, G. W. and Daroszewska, M. (1996) Deuterated vitamin E: measurement in tissues and body fluids, in Free Radicals: A Practical Approach (Punchard, N. A. and Kelly, F. J., eds.), Oxford University Press, Oxford, pp. 257–270.Google Scholar
  17. 17.
    Hatch, F. T. and Lees, R. S. (1968) Practical methods for plasma lipoprotein analysis, in Advances in Lipid Research (Paoletti, R. and Kritchevsky, D., eds.), Academic Press, New York, pp. 2–68.Google Scholar
  18. 18.
    Lindgren, F. T., Jensen, L. C., and Hatch, F. T. (1972) The isolation and quantitative analysis of serum lipoproteins, in Blood Lipids and Lipoproteins: Quantification, Composition, and Metabolism (Nelson, G. S., ed.), John Wiley and Sons, New York, NY, pp. 181–274.Google Scholar
  19. 19.
    Ganong, W. F. (1987) Review of Medical Physiology 13. Appleton & Lange, Norwalk, CT.Google Scholar
  20. 20.
    Moore, F. D., Oleson, K. H., McMurrey, J. D, Parker, H. V., Ball, M. R., and Boyden, C. M. (1963) The body cell mass and its supporting environment, in Body Composition in Health and Disease, W.B. Saunders, Philadelphia, p. 67.Google Scholar
  21. 21.
    Ryan, W. G. and Schwartz, T. B. (1965) Dynamics of plasma triglyceride turnover in man. Metabolism 14, 1243–1254.CrossRefPubMedGoogle Scholar
  22. 22.
    Clifford, A. J. and Muller, H-G. (1998) in Mathematical Modeling in Experimental Nutrition. 1. Plenum Press, New York.Google Scholar
  23. 23.
    Parks, E. J. and Traber, M. G. (2000) Mechanisms of vitamin E in aging. Antioxid. Redox Signal., 2, 405–412.CrossRefPubMedGoogle Scholar
  24. 24.
    Parks, E. J., Dare, D., Frazier, K., Hughes, E., Hellerstein, M. K., Neese, R. A., and Traber, M. G. (2000) Dependence of plasma α-tocopherol flux on very low density lipoprotein triglyceride clearance in humans. Free Rad. Biol. Med., 29, 1151–1159.CrossRefPubMedGoogle Scholar

Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Elizabeth J. Parks
    • 1
  1. 1.Department of Food Science and NutritionUniversity of MinnesotaTwin Cities, St. Paul

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