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Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC

  • Richard W. Browne
  • Donald Armstrong
Part of the Methods in Molecular Biology™ book series (MIMB, volume 186)

Abstract

Lipid peroxidation (LPO) is a prominent manifestation of free radical (FR) activity in biological systems. The primary target of FR attack on lipids is the 1,4-pentadiene structure of a polyunsaturated fatty acid (PUFA), which are either free or esterified to cholesterol or glycerol. Initiation occurs when a FR abstracts a methylene hydrogen from PUFA. In this reaction the FR is quenched and a PUFA centered alkoxyl radical (L•) is formed. L• then undergoes a spontaneous rearrangement of its double bonds forming a conjugated diene. Reaction of L• with molecular oxygen produces a PUFA-centered peroxyl radical (LOO•). Propagation occurs when either L• or LOO• act as initiating FR and attack a neighboring PUFA in a tightly packed lipid bilayer structure of a membrane or within a lipoprotein. The product of this reaction is a new L•, which can further propagate the reaction and form a lipid hydroperoxide (LHP) (1). Termination occurs when an antioxidant (AOX) molecule capable of absorbing the intermediate free radicals, or free-radical scavengers, interrupts this chain reaction.

Keywords

Micron Particle Size Free Radical Attack Eicosatetraenoic Acid Shimadzu Scientific Instrument Intermediate Free Radical 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2002

Authors and Affiliations

  • Richard W. Browne
    • 1
  • Donald Armstrong
    • 2
  1. 1.Department of Biotechnology and Clinical Laboratory ScienceState University of New YorkNYUSA
  2. 2.Free Radical and Antioxidant Laboratory and Department of Small Animal Clinical SciencesUniversity of Florida, College of Veterinary MedicineGainesville

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