Abstract
The preparation of fixed-concentration polyacrylamide gels has been described in Chapters 10 and 11. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate until the decreasing pore size impedes further progress. Once the ”pore limit“ is reached, the protein banding pattern does not change appreciably with time, although migration does not cease completely (1). There are two main advantages of gradient gels over linear gels.
Similar content being viewed by others
Reference
Margolis, J. and Kenrick, K. G. (1967) Nature (London), 214, 1334.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2002 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Walker, J.M. (2002). Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:69
Download citation
DOI: https://doi.org/10.1385/1-59259-169-8:69
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive