O-Linked Oligosaccharide Profiling by HPAEC-PAD

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith

Abstract

Although it can often be an advantage to be able to chromatograph neutral and sialylated oligosaccharides/alditols in one run (see  Chapter 114), the added resolution of HPAEC and sensitivity with PAD detection means that this is an additional desirable technique for analysis. Neutral oligosaccharide alditols are poorly retained on CarboPac PA1, but can be resolved on two consecutive columns (1). The carboPac PA1 column is ideal for sialylated oligosaccharides (2) and can also be used for sialylated alditols.

Keywords

Titanium HPLC Hydrate Helium H2SO4 

References

  1. 1.
    Campbell, B. J., Davies, M. J., Rhodes, J. M., and Hounsell, E. F. (1993) Separation of neutral oligosacharide alditols from human meconium using high-pH anion-exchange chromatography. J. Chromatogr. 622, 137–146.PubMedCrossRefGoogle Scholar
  2. 2.
    Lloyd, K. O. and Savage, A. (1991) High performance anion exchange chromatography of reduced oligosaccharides from sialomucins. Glycoconjugate J. 8, 493–498.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
  • Kevin D. Smith
  1. 1.School of Biological and Chemical SciencesBirkbeck University of LondonUK

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