O-Linked Oligosaccharide Profiling by HPAEC-PAD
Although it can often be an advantage to be able to chromatograph neutral and sialylated oligosaccharides/alditols in one run (see Chapter 114), the added resolution of HPAEC and sensitivity with PAD detection means that this is an additional desirable technique for analysis. Neutral oligosaccharide alditols are poorly retained on CarboPac PA1, but can be resolved on two consecutive columns (1). The carboPac PA1 column is ideal for sialylated oligosaccharides (2) and can also be used for sialylated alditols.
KeywordsTitanium HPLC Hydrate Helium H2SO4