Staining of Glycoproteins/Proteoglycans in SDS-Gels
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of molecular weight determinations. In this chapter, staining methods are described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.
KeywordsAlcian Blue Periodic Acid Molecular Weight Determination Silver Solution Automatic Staining
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