Staining of Glycoproteins/Proteoglycans in SDS-Gels

  • Holger J. Møller
  • Jørgen H. Poulsen

Abstract

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of molecular weight determinations. In this chapter, staining methods are described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.

Keywords

Formaldehyde Glycerol Carbohydrate Electrophoresis Dodecyl 

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Holger J. Møller
    • 1
  • Jørgen H. Poulsen
    • 1
  1. 1.Department of Clinical BiochemistryAarhus University HospitalAarhusDenmark

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