Mass Spectrometric Analysis of Protein Phosphorylation

  • Débora Bonenfant
  • Thierry Mini
  • Paul Jenö


Phosphorylation is one the most frequently occurring posttranslational modifications in proteins, playing an essential role in transferring signals from the outside to the inside of a cell and in regulating many diverse cellular processes such as growth, metabolism, proliferation, motility, and differentiation. It is estimated that up to one third of all proteins in a typical mammalian cell are phosphorylated (1). Phosphorylation is carried out by a vast group of protein kinases which are thought to constitute 3% of the entire eukaryotic genome (1-3). To decipher the recognition signal of protein kinases and protein phosphatases acting on a given molecular target, and to understand how the activity of the target protein is regulated by phosphorylation, it is important to define the sites and the extent of phosphorylation at each specific site.


Phosphorylation Site Okadaic Acid Neutral Loss Calf Intestinal Alkaline Phosphatase Micropipet Puller 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Débora Bonenfant
    • 1
  • Thierry Mini
    • 1
  • Paul Jenö
    • 1
  1. 1.Department of BiochemistryBiozentrum der Universität BaselSwitzerland

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