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Autoradiography and Fluorography of Acrylamide Gels

  • Antonella Circolo
  • Sunita Gulati

Abstract

Autoradiography detects the distribution of radioactivity on gels or filters by producing permanent images on photographic film. It is frequently used in a variety of experimental techniques ranging from Southern and Northern blot analysis (1), to visualization of radioactive proteins separated in a sodium dodecyl sulfate (SDS)-Poly-acrylamide gels (2), to detection of nuclear factors bound to a labeled DNA probe in gel shift analysis (3), and to localization of DNA bands in sequencing gels (4).

Keywords

Whatman Paper Acetic Acid Glacial Plastic Wrap Cupric Sulfate Radioactive Band 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Antonella Circolo
    • 1
  • Sunita Gulati
    • 1
  1. 1.Maxwell Finland Lab for Infectious DiseasesBoston

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