Advertisement

Antigen Measurement Using ELISA

  • William Jordan

Abstract

The initial description of the enzyme-linked immunosorbent assay (ELISA) almost 30 yr ago (1) marked a technological advance that has had an immense impact in both clinical diagnostic and basic scientific applications. This assay represents a simple and sensitive technique for specific, quantitative detection of molecules to which an antibody is available. Although there are a huge number of variations based on the original ELISA principle, this chapter focuses on perhaps the two most useful and routinely performed: (1) the indirect sandwich ELISA/3-providing high sensitivity and specificity and (2) the basic direct ELISA/3-useful when only one antibody to the sample antigen is available.

Keywords

Fetal Calf Serum Detection Antibody Indirect ELISA Capture Antibody Coating Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Engvall, J. R. and Perlamann, P. (1971) Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8, 871–874.PubMedCrossRefGoogle Scholar
  2. 2.
    Roberts, I. M., Solomon, S. E., Brusco, O. A., Goldberg, W., and Bernstein, J. J. (1991) A comparison of the sensitivity and specificity of enzyme immunoassays and time-resolved fluoromimmunoassay. J. Immunol. Meth. 143, 49–56.CrossRefGoogle Scholar
  3. 3.
    Self, C. H. (1985) Enzyme amplification/3-a general method applied to provide an immunoassisted assay for placental alkaline phosphatase. J. Immunol. Meth. 76, 389–393.CrossRefGoogle Scholar
  4. 4.
    Bronstein, I., Voyta, J. C., Thorpe, G. H., Kricka, L. J., and Armstrong, G. (1989) Chemiluminescent assay of alkaline phosphatase applied in an ultrasensative enzyme immunoassay of thyrotropin. Clin. Chem. 35, 1441–1446.PubMedGoogle Scholar
  5. 5.
    Cook, D. B. and Self, C. H. (1993) Determination of one-thousandth of an attomole (1 zeptomole) of alkaline phosphatase: application in an immunoassay of proinsulin. Clin. Chem. 39, 965–971.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • William Jordan
    • 1
  1. 1.Department of Immunology, ICSMHammersmith HospitalLondonUK

Personalised recommendations