Advertisement

Purification of IgG Using Thiophilic Chromatography

  • Mark Page
  • Robin Thorpe

Abstract

Immunoglobulins recognize sulphone groups in close proximity to a thioether group (1), and, therefore, thiophilic adsorbents provide an additional chromatographic method for the purification of immunoglobulins that can be carried out under mild conditions preserving biological activity. A thiophilic gel is prepared by reducing divinylsulfone (coupled to Sepharose 4B) with β-mercaptoethanol. The product is of intermediate purity and would be useful for further processing, e.g., purification by ion-exchange, size exclusion, and/or affinity chromatography.

Keywords

Sodium Azide Binding Buffer Sodium Carbonate Ascitic Fluid Vacuum Filtration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Porath, J., Maisano, F., and Belew, M. (1985) Thiophilic adsorption-a new method for protein fractionation. FEBS Lett. 185, 306–310.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

Personalised recommendations